The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 30 Issue 1
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- Pages.61-70
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- 2000
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- 1225-2344(pISSN)
Comparative Study of the Standard Plaque Assay with Solid-overlay and Immunofocus Assay for Varicella-zoster Virus Titration
수두바이러스의 정량에 있어서 Solid-overlay Standard Plaque Assay와 Immunofocus Assay의 효용성 비교 연구
- Lee, Hwa-Kyung (Molecular Virology Laboratory, Department of Biology, College of Liberal Arts and Sciences, Kyung Hee University) ;
- Jeong, Yong-Seok (Molecular Virology Laboratory, Department of Biology, College of Liberal Arts and Sciences, Kyung Hee University)
- Published : 2000.03.30
Abstract
Standard plaque assay using agarose-overlay has long been used for titration of many infectious virus particle. Plaque assay for the titration of varicella-zoster virus and its live vaccine requires three intermittent agarose overlay to visualize plaques. Overall procedure of the assay takes at least nine days from virus inoculation and microbe contamination including fungi is frequently accompanied during incubation period. We studied whether an immunofocus assay in conjunction with peroxidase-mediated immunohistochemical reaction may replace the standard plaque assay for the virus titration by comparing the two methods. A linear relationship was observed between number of foci and virus dilution. The number of foci in a given dilution of virus appeared a little higher than counted plaques formed in standard plaque assay. Independent titration results obtained from two assay methods for a given dilution of virus demonstrated a strong correlation (