한탄바이러스 Nucleocapsid Protein 발현에 있어 S Genome 내 Noncoding Region의 역할

The Role of Noncoding Region in Hantaan Viral S Genome for Expression of Nucleocapsid Protein

  • 유정희 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 이연승 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 이호동 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 박찬 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 박근용 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 이평우 (고려대학교 의과대학 미생물학교실)
  • Yu, Cheong-Hee (Division of Arbovirus, Department of Virology, National Institute of Health) ;
  • Lee, Yeon-Seung (Division of Arbovirus, Department of Virology, National Institute of Health) ;
  • Lee, Ho-Dong (Division of Arbovirus, Department of Virology, National Institute of Health) ;
  • Park, Chan (Division of Arbovirus, Department of Virology, National Institute of Health) ;
  • Park, Keun-Yong (Division of Arbovirus, Department of Virology, National Institute of Health) ;
  • Lee, Pyung-Woo (Department of Microbiology, College of Medicine, Korea University)
  • 발행 : 2000.03.30

초록

The genome of Hantaan virus, the prototype of the hantavirus genus, is composed of three segmented, single stranded negative sense RNA genome. The 5' and 3' termini of the Hantaan virus RNA genome contain noncoding regions (NCRs) that are highly conserved and complementary to form panhandle structures. There are some reports that these NCRs seems to control gene expression and viral replication in influenza virus and vesicular stomatitis virus. In this study, we examined whether NCRs in Hantaan virus playa role in expression of the viral nucleocapsid protein (Np) and foreign (luciferase) gene. The 5' and/or 3' NCR-deleted mutants were constructed and analysed. The Np expression of 5' NCR-deleted clone was similar to that of the clone containing full S genome. In the case of 3' NCR-deleted clone, it showed 40% reduction. To investigate the role of NCR in foreign gene expression, the clones which are replaced ORF of Hantaan viral Np gene with that of luciferase gene were constructed. The results were similar to those of the experiments using Np gene. These results suggest that 3' NCR is more important than 5' NCR in protein expression. To find out a critical region of 3' NCR in protein expression, several clones with a deleted part of 3' NCR were constructed and analyzed. The deletion of the conserved region in 3' NCR showed $20{\sim}30%$ decrease in Np expression. However there were no change in luciferase activities between clones with or without non-conserved region of 3' NCR. These results suggest that the 3' NCR of Hantaan virus S genome, especially conserved region in 3' NCR, plays an important role in the expression of Hantaan viral Np and foreign genes.

키워드