DOI QR코드

DOI QR Code

Kinetic Studies of Aspartase from Hafnia alvei byTemperature Dependence Activity Changes


초록

The temperature dependence of the kinetic parameters of the aspartase-catalyzed reaction has been examined in the direction of deamination. The pK1values at 37$^{\circ}C$, 25$^{\circ}C$, 16$^{\circ}C$ and 7$^{\circ}C$ were 6.2 $\pm$ 0.1, 6.3 $\pm$ 0.1, $6.7{\pm}0.3$ and 6.9 $\pm$ 0.3, respectively. On the other hand, the pK2 values at 37$^{\circ}C$,25$^{\circ}C$, 16$^{\circ}C$ and 7$^{\circ}C$ were 8.1 $\pm0.2$, 8.3 $\pm$ 0.2, 8.2 $\pm$ 0.3 and 8.0 $\pm$ 0.2,respectively. The enthalpy of ionization, DHion, calculated from the slope of pK1, are 6.0 $\pm$ 0.3 kcal/mol. These results validate the prediction that aspartase requires a histidine residue for a general base, and a cysteine (or having a carboxyl functional group) for a general acid.

키워드

참고문헌

  1. Biochem. J. v.20 no.545 Quastel, J. H.;Woolf, B.
  2. Arch. Biochem. Biophys. v.287 no.60 Karsten, W. E.;Viola, R. E.
  3. Biochemistry v.7 no.3403 Bada, J. L.;Miller, S. L.
  4. J. Biol. Chem. v.242 no.2973 Williams, V. R.;Lartigue, D. L.
  5. Biochemistry v.23 no.5168 Nuiry, I. I.;Hermes, J. D.;Weiss, P. M.;Chen, C.-Y.;Cook, P. F.
  6. Arch. Biochem. Biophys. v.320 no.115 Yoon, M. Y.;Thayer-Cook, K. A.;Berdis, A. J.;Karsten, W. E.;Schnackerz, K. D.;Cook, P. F.
  7. Adv. in Enzymology v.45 no.273 Cleland, W. W.
  8. Bull. Korean Chem. Soc. v.19 no.31 Yoon, M. Y.;Cho, C. G.
  9. Biophycal Chemistry v.V Edsall, J. T.;Wyman, J. C.
  10. J. Biochem. Mol. Biol. v.30 no.113 Yoon, M. Y.;Park, J. H.;Choi, K. J.;Kim, J. M.;Kim, Y. O.;Park, J. B.;Kyung, J. B.
  11. J. Biochem. Mol. Biol. v.30 no.113 Shim, J. B.;Kim, J. S.;Yoon, M. Y.
  12. J. Biochem. Mol. Biol. v.32 no.326 Shim, J. B.;Kim,H. J.;Yoon, M. Y.
  13. Ida, N.; Tokushige, M. J. Biochem. v.98 no.793 Ida, N.;Tokushige, M.
  14. Biochem. Biophys. Acta v.403 no.221 Mituta, K.;Tokushike, M.
  15. Biochem. Biophys. Res. Commun. v.177 no.414 Murase, S.;Takagi, J. S.;Higashi, Y.;Imaishi, H.;Yumoto, N.;Tokushige, M.
  16. J. Biol. Chem. v.269 no.6313 Saribas, A. S.;Schindler, J. F.;Viola, R. E.
  17. Arch. Biochem. Biophys. v.341 no.329 Giorgianni, F.;Beranova, S.;Wesdemiotis, C.;Vila, R. E.
  18. Biochemistry v.36 no.9145 Jayasekara, M. M.;Shi, W.;Farber, G. K.;Viola, R. E.
  19. J. Biochem. Mol. Biol. v.32 no.572 Lee, M. S.;Choi, K. J. Kwon, S. J.;Kang, I.;Ha, J.;Kim, S. S.;Han, M. S.;Yoon, M. Y.
  20. Method in Enzymology v.63 Cleland, W. W.

피인용 문헌

  1. Reversible Denaturation and Reactivation of Aspartase from Hafnia alvei vol.29, pp.1, 2000, https://doi.org/10.5012/bkcs.2008.29.1.208