Bulletin of the Korean Chemical Society

  • 제21권12호
  • /
  • Pages.1171-1172
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  • 2000
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Replication Protein A (RPA) Regulates Its DNA Binding Activity through Redox

  • 발행 : 20001200
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초록

키워드

참고문헌

  1. EMBO J. no.7 Fairman, M. P.;Stillman, B.
  2. Nature no.342 Brill, S. J.;Stillman, B.
  3. Bio-chemistry no.32 Mitsis, P. G.;Kowalczykowski, S. C.;Lehman, I. R.
  4. Mol. Biochem. Parasitol. no.59 Brown, G. W.;Melendy, T.;Ray, D. S.
  5. Proc. Natl. Acad. Sci. USA no.85 Wold, M. S.;Kelly, T. J.
  6. EMBO J. no.13 Carty, M. P.;Zernik-Kobak, M.;McGrath, S.;Dixon, K.
  7. J. Biol. Chem. no.274 Park, J-S.;Park, S-J.;Peng, X.;Wang, M.;Yu, M-A.;Lee, S.-H
  8. J. Biol. Chem. no.274 Park, J-S.;Wang, M.;Park, S-J.;Lee, S-H.
  9. Yeast wild-type RPA and human mutant RPA were puri-fied according to the Reference 2 and 8, respectively.
  10. RPA-ssDNA binding assay: Oligo $(dT)_{50}$was 5'-end label-ed with $[γ^32P]$ATP (Du Pont) and T4 polynucleotide kin-ase (USB) based on the manufacturer’s instructions. The indicated amount of wild-type or mutant RPA was incu-bated with 100 fmol of $5'-^32$P-labeled Oligo $(dT)_{50}$ at room temperature for 15 min in the reaction mixtures (30 mL) containing 50 mM Hepes-KOH (pH 7.8), 10 mM $MgCl_2$ polydI : dC (0.2 mg), BSA (0.2 μg/μL), and indicated amounts of DTT or NaDI. Protein-DNA complexes were analyzed using 5% polyacrylamide gels in 1x TBE buffer (acrylamide: bisacrylamide = 79 : 1), The gels were dried and exposed to x-ray films (Kodak), The bands of interest were excided from the gels and measured for radioactivity using a Beckman Scintillation Counter LS 6500
  11. Science no.249 Abate, C.;Patel, L.;Rauscher III, F. J.;Curran, T.
  12. J. Biol. Chem. no.271 Nakshatri, H.;Bhat-Nakshatri, P.;Currie, R. A.
  13. Genes & Dev. no.11 Jayaraman, L.;Murthy, K. G. K.;Zhu, C.;Curran, T.;Xanthoudakis, S.;Curran, T.
  14. J. Biol. Chem. no.273 Tell, G.;Scaloni, A.;Pellizzari, L.;Formisano, S.;Pucillo, C.;Damante, G.
  15. EMBO J. no.11 Xanthoudakis, S.;Curran, T.
  16. Plasmid constructs and site-directed mutagenesis: cDNA encoding the p70 subunit ofhuman RPA was cloned into baculovirus transfer vector pVL941-SW. For the change Cys-289’ Ala, the plasmid pVL941SW-70 (carring wild-type p70 gene) and the fellowing primers were used in the PCR reaction, with the underlined nucleotides changes from the wild-type sequence: 5-TCC GTC ATG CCC GCC GAG GAC GAC CAT-3 and 5-ATG GTC GTC CTC GGC GGG CAT GAC GGA-3. The mutation procedure was performed according to the Stratagene protocol.
  17. Science no.282 Clarke, N. D.;Berg, J. M.
  18. J. Biol. Chem. no.266 Hutchison, K. A.;Matic, G.;Meshinchi, S.;Bresnick, E. H.;Pratt, W. B.
  19. Science no.281 Gowen, L. C.;Avrutskaya, A. V.;Latour, A. M.;Koller, B. H.;Leadon, S. A.
  20. Mol. Cell. Biol. no.16 Wu, X.;Bishopric, N. H.;Discher, D. J.;Murphy, B. J.;Webster, K. A.
  21. Nature no.352 Luisi, B. F.;Xu, W. X.;Otwinowski, Z.;Freedman, L. P.;Yamamoto, K. R.;Sigler, P. B.
  22. J. Inorg. Biochem. no.71 Casadevall, M.;Sarkar, B.

피인용 문헌

  1. Identification of the Structural Change of Human Replication Protein A (hRPA) in the ssDNA Binding and Redox Potential vol.26, pp.8, 2000, https://doi.org/10.5012/bkcs.2005.26.8.1168