Effects of Radix Angelicae Gigantis and Resina Ferulae on the Relaxation of Smooth Muscle and Expression of iNOS

당귀 및 아위가 평활근 이완과 iNOS 발현에 미치는 영향

  • 김성재 (원광대학교 한의과대학 신계내과학교실) ;
  • 송봉근 (원광대학교 한의과대학 신계내과학교실) ;
  • 이언정 (원광대학교 한의과대학 신계내과학교실) ;
  • 김형균 (원광대학교 한의과대학 신계내과학교실) ;
  • 김중길 (원광대학교 한의과대학 신계내과학교실)
  • Published : 2000.06.01

Abstract

Objectives : Radix Angelicae Gigantis(RAG) and Resina Ferulae(RF) have been used in oriental medicine or folk medicine to increase stamina. The aim of this study was the characterization of the mechanism of action of RAG and RF on smooth muscle and macrophages in rats to find new substances for the treatment of erectile dysfunction, cardiovascular diseases and immune dysfunction. Methods : We investigated the effects of the water extracts of RAG and RF on phenylephrine or KCl-contracted rat endothelium-denuded aorta, the production of NO in vascular smooth muscle cell (VSMC) and the production of NO and induction of iNOS in the $IFN-{\gamma}-primed$ RAW 264.7 cells. Results : The water extracts of the RAG and RF showed significant concentration-dependent relaxation effects on phenylephrine or KCl-contracted rat endothelium-denuded aorta. It also reduced the tension of the rat endothelium denuded aorta which was contracted in $Ca^{2+}-free$ media. On the other hand, it increased production of NO in VSMC which was stimulated with $IL-{\beta}$ or $IL-{\beta}$ plus $IFN-{\gamma}$. The water extracts of RAG and RF increased production of NO and induction of iNOS in the $IFN-{\gamma}-primed$ RAW 264.7 cells. Conclusions : According to the above results, the water extracts of RAG and RF relaxed the smooth muscle effectively and increased the production of NO in VSMC and macrophages. So, these herbs can be applied to erectile dysfunction, hypertension, angina pectoris, artherosclerosis and a defense defect for virus or microbe.

Keywords

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