The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Protective Effects of Woowhangcheongshim-won(WCW) on Middle Cerebral Artery Occlusion

우황청심원이 뇌허혈을 유발시킨 흰쥐의 신경전달물질에 미치는 영향

  • 박치상 (경산대학교 한의과대학 심계내과학교실) ;
  • 이은주 (경산대학교 한의과대학 심계내과학교실) ;
  • 박원미 (경산대학교 한의과대학 약리학교실) ;
  • 김미려 (경산대학교 한의과대학 약리학교실) ;
  • 조정숙 (동국대학교 의과대학 약리학교실) ;
  • 김영호 (충남대학교 약학대학 생약학교실) ;
  • 양재하 (경산대학교 한의과대학 약리학교실) ;
  • 박창국 (경산대학교 한의과대학 심계내과학교실)
  • Published : 2000.03.01
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Abstract

The present study was carried out to investigate the effects of Woowhangcheongshim-won(WCW) on the extracellular concentrations of amino acid neurotransmitters(glutamate, aspartate, GABA, glycine, taurine, alanine, and tyrosine) and organic acid (lactate and pyruvate) in striatum and cerebral infarction volume in rats subjected to permanent focal cerebral ischemia induced by 2 hours of middle cerebral artery occlusion(MCAO), using intracerebral microdialysis as the sampling technique, Microdialysis probes were inserted into the lateral part of the caudate-putamen 2 hours before the experiment and microdialyzates were collected at 20min intervals and analyzed by high performance liquid chromatography, WCW significantly decreased the infarction volume with reducing focal cerebral ischemia-induced increase of extracellular glutamate, asparate, and tyrosine. On the other hand, the increase of GABA and taurine was enhanced after treatment of WCW in the ischemia-induced rats, These results suggest that WCW can produce a neuroprotective effect against cerebral ischemia by regulating extracellular excitatory and inhibitory amino acid levels in relation to the concept of excitotoxicity in brain ischemia.

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References

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