Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 24 Issue 4
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- Pages.407-417
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- 2000
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- 1226-5284(pISSN)
In Vitro Development of Bovine Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts
태아 섬유아세포로 재구성된 핵치환 소 수정란의 체외발달
- Koo, D.B. (Korea Research Institute of Bioscience and Biotechnology) ;
- Choi, Y.H. (Korea Research Institute of Bioscience and Biotechnology) ;
- Park, J.S. (Korea Research Institute of Bioscience and Biotechnology) ;
- Kim, H.N. (Korea Research Institute of Bioscience and Biotechnology) ;
- Kang, Y.K. (Korea Research Institute of Bioscience and Biotechnology) ;
- Lee, C.S. (Korea Research Institute of Bioscience and Biotechnology) ;
- Han, Y.M. (Korea Research Institute of Bioscience and Biotechnology) ;
- Park, H.D. (Department of Biotechnology, Taegu University) ;
- Lee, K.K. (Korea Research Institute of Bioscience and Biotechnology)
- 구덕본 (한국생명공학연구원) ;
- 최영희 (한국생명공학연구원) ;
- 박정선 (한국생명공학연구원) ;
- 김하나 (한국생명공학연구원) ;
- 강용국 (한국생명공학연구원) ;
- 이철상 (한국생명공학연구원) ;
- 한용만 (한국생명공학연구원) ;
- 박흠대 ;
- 이경광 (한국생명공학연구원)
- Published : 2000.12.01
Abstract
The present study was to examine effects of various electrical stimulus treatments used for electro-fusion on the preimplantation development of bovine nuclear transfer (NT) embryos with fetal fibroblast cells. Fetal fibroblast cells were isolated from one fetus at day 45 of gestation in Holstein cow, and passaged 3 to 4 times before being transferred into enucleated oocytes. Single fibroblast cells were individually placed into the perivitelline space of enucleated oocytes by using a micromanipulator. At first, the fusion and developmental rates of reconstructed oocytes were compared between different electric stimulation conditions. When fusion of the reconstructed oocyte was induced by different electric pulse periods (15, 30 and 45
본 연구는 태아 섬유아세포를 이용한 소 핵치환 난자의 융합에 있어서 여러 조건의 전기자극 처리 후 그에 따른 발달율을 조사하였다. 임선 45 일령 태아로부터 섬유아세포를 분리하여 3~4 차례 계대배양후, 이들 단일 섬유아 세포들을 미세조작기를 이용하여 제핵된 난자의 위란강 내에 주입하였다. 본 실험에서는 첫째로, 다른 전기자극 조건의 적용에 따른 융합율 및 발달율을 비교 조사하였다. 180 V/mm 의 조건하에서 다른 조건의 전기자극 시간 (15, 30, 45