Abstract
The present study was carried out in order to investigate the effects of antler extract on hormone concentration, Ca, P and alkaline phosphatase (ALP) levels in ovariectomized rats. Rats were ovariectomized bilaterally and were fed up with Ca- and P-free diet in order to induce osteoporosis. Osteoporosis was determined by the extent of density of bone mineral and lowering the concentrations of serum Ca and P. Male or female antler extract were administrated for 5 weeks to elucidate the protective and therapeutic effects for osteoporosis. The serum concentrations of estradiol, progesterone, calcitonin, osteocalcin, Ca and P, and the activities of ALP of ovariectomized rats were examined for 5 weeks. 1. After administration of female or male antler extract to osteoporosis-induced rats at the doses of 625 mg/kg and 1,250 mg/kg, respectively, the levels of the serum estradiol of the ovariectomized rat significantly decreased from 40.50$\pm$3.34 pg/$m\ell$ to 20.80$\pm$1.86 pg/$m\ell$ for 5 weeks, the levels of serum estradiol were a signigicant lower than those of control group (49.50$\pm$2.70~50.80$\pm$3.l3 pg/$m\ell$). 2. After administration of female or male antler extract to osteoporosis-induced rats at the doses of 625 mg/kg and 1,250 mg/kg, respectively, the levels of serum progesterone were didn't show significant differences. 3. After administration of female or male antler extract to osteoporosis-induced rats at the doses of 625 mg/kg and 1,250 mg/kg, respectively, the levels of serum calcitonin were a little higher than those of control group (0.64$\pm$0.03 ~0.68$\pm$0.04 pg/$m\ell$). 4. After administration of female or male antler extract to osteoporosis-induced rats at the doses of 625 mg/kg and 1,250 mg/kg, respectively, the levels of serum osteocalcin were little higher than those of control groups (0.28$\pm$0.02~0.31$\pm$0.02 ng/$m\ell$). In addition, the levels of serum osteocalcin of female antler extract administered group were little higher than those of male antler extract administered group. 5. The levels of serum Ca and P in osteoporosisinduced rats, administered with male or female antler extract, were little higher than those of control group. However, the levels of serum Ca and P in ovariectomized rats were significantly higher than those of control group (p＜0.05). 6. After administration of female or male antler extract to osteoporosis-induced rats, the activities of serum ALP increased compared with those of normal control group. There were significant differences between the serum ALP activities of FA 1,250 and 625 groups (p＜0.05). These findings suggest a possible protective and therapeutic effects of female or male antler extract against bone loss in ovariectomized rats, associated with a significant increase of serum estradiol level.
본 연구는 골다공증유발 rat에 녹용추출물의 투여가 예방 및 치료효과에 미치는 영향을 구명하고자, 양측 난소를 적출한 후 골다공증이 유발된 rat에 암, 수녹용 추출물 625, 1,250 mg/kg을 5 주간 투여하면서 혈청중 estradiol, progesterone, calcitonin, osteocalcin 농도와 Ca, P 및 ALP 함량변화등을 조사하였다. 1. 난소적출 후 골다공증이 유발된 rat에 암, 수녹용 추출물 625mg/kg, 1,250 mg/kg을 각각 투여했을 때 혈청내 estradiol 수준은 난소적출군이 20.80$\pm$1.86~40.50$\pm$pg/$m\ell$로서 정상대조군의 49.50 :t2. 70-50.80 :t3.13 pg/$m\ell$에 비하여 유의한 감소를 나타냈다 (p<0.05). 2. 골다공증 유발 rat 에 암 수녹용 추출물 625 mg/kg, 1,250 mg/kg을 각각 투여했을 때 혈청중 progesterone 수준은 정상대조군의 50.90$\pm$3.63~53.40$\pm$2.74 ng/$m\ell$에 비하여 큰 차이를 나타내지 않았다. 3. 골다공증 유발 rat 에 암, 수녹용 추출물 625 mg/kg, 1,250 mg/kg을 투여했을 때 혈청중 calcitonin 수준은 정상대조군의 0.64$\pm$0.03~0.68$\pm$0.04 pg/$m\ell$에 비 하여 약간 높은 증가를 나타냈으며 특히, 난소적출군은 대조군에 비해 현저한 감소를 나타냈다. 4. 골다공증 유발 rat 에 암, 수녹용 추출물 625 mg/kg, 1,250 mg/kg을 각각 투여했을 때 혈청중 osteocalcin 수준은 정상대 조군의 0.28$\pm$0.02 ~0.31$\pm$0.02 ng/$m\ell$에 비하여 약간 높은치를 나타냈다. 한편, 암, 수녹용 추출물의 처리군의 osteocalcin 치는 암컷녹용 추출물처리군이 약간 높았으나 유의성은 인정되지 않았다. 5. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 혈청중 Ca, P 함량은 각각 정상대조군에 비하여 약간 높은 치를 나타냈으며, 난소적출 무처치군은 대조군에 비해 유의한 증가를 나타냈다 (p＜0.05). 6. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 혈청중 ALP 함량은 정상대조군에 비하여 증가치를 나타냈으며, 암, 수사슴녹용 추출물의 처리군중 대조군과 FA1,250 처리군과 MA625 처리군간에 유의한 변화가 인정되었다 (p＜0.05). 위의 결과들에서 암, 수녹용 추출액은 난소제거후의 estrogen의 감소를 억제시키고 골소실을 억제하므로 골다공증의 예방과 치료에 효과가 있는 것으로 판단된다.