Toxicological Research
- Volume 16 Issue 3
- /
- Pages.201-209
- /
- 2000
- /
- 1976-8257(pISSN)
- /
- 2234-2753(eISSN)
Uterotrophic Activity of Ethinyl Estradiol by Gavage and Subcutaneous Administration in Immature Female Rats
미성숙 랫드에 있어서 경구 및 피하투여에 의한 Ethinyl estradiol의 자궁증식효과
Abstract
In association with the international validation program to establish a rodent uterotrophic assay, we conducted preliminary uterotrophic assay proposed by GECD using immature female rats. In the present study, oral and subcutaneous routes were chosen to compare the effects of estrogenic com-pounds in the two dosing regimens. The reference compound ethinyl estradiol (EE) and the antagonist ZM189154(ZM) were administered by gavage or subcutaneously (s.c.) to immature female SD rats from 20 to 22 days of age. For each study, sixty-six female rats were randomly assigned to eleven groups: Untreated control, EE 0,0.01, 0.03, 0.1, 0.3, 1.0,3.0 and 10.0
Keywords
- Endocrine disrupting chemicals;
- In vivo screening assay;
- Ethinyl estradiol;
- Estrogen;
- Uterus weight;
- Immature female rat