Abstract
In this study, we observed the effects of dioxin on weights of body, testes and other organs, the number and motility of sperm in the a various dose after two days' of administration in mice. Animals were treated with oral dose of dioxin 10, 20, 30, 40 mg/kg body weight, respectively. 1, After administration dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the changes in body weights were 30.6 $\pm$ 2.g~40.7 $\pm$ 3.9g and 30.8 $\pm$4.1g~39.5g $\pm$3.1 for 10 and 20 $\mu\textrm{g}$/kg dosed group, 31.0 $\pm$ 3.5g ~ 39.0 $\pm$ 3.5g, 30.6 $\pm$ 3.4g~38.3 $\pm$ 4.0g for 30 and 40 $\mu\textrm{g}$/kg dosed group. The body weight of dioxin-administered group showed lower value when compared to 30.6 $\pm$ 2.8g ~ 44.5 $\pm$ 3.1g of which is control group's. 2. After administration of dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the increase in the number of WBC was prominent, but the increase in the number of RBC wasn't significant, though the values of Hb, PCV, and PLT were higher than those of control group's. 3. After administration of dioxin at doses of 10. 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the changes in sperm number were 112.5 $\pm$ 3.7~119.4 $\pm$4.2 $\times$ 10$^{6}$ $m\ell$, 103.9 $\pm$3.8 ~ 110.2 $\pm$ 3.6 $\times$ 10$^{6}$ $m\ell$, 97.5 $\pm$ 3.4 ~105.7 $\pm$ 4.4 $\times$ 10$^{6}$ $m\ell$, 87.2 $\pm$ 3.7~98.5 $\pm$ 3.8 $\times$ 10$^{6}$ $m\ell$, respectively. The sperm number of dioxin-administered group showed lower value than that of control group's, which was 119.0 $\pm$ 4.3 ~ 120.7 $\pm$ 4.8 $\times$ 10$^{6}$ $m\ell$. After administration of dioxin at doses of 10~40 $\mu\textrm{g}$/kg to the mice, the sperm motility were 69.4$\pm$ 3.0 ~ 86.6 $\pm$4.7%. The sperm motility of dioxin-administered group showed lower value than that of control group's. 4. After administration of dioxin at doses of 10, 20, 30 and 40$\mu\textrm{g}$/kg to the mice, the organ weight of each dioxin-administered group's was decreased a little compared to that of control group's. 5. After administration of dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the weights of spleen, kidneys, and liver showed increase a little.
본 연구는 dioxin이 생체에 미치는 영향을 구명하고자 dioxin 10~40 $\mu\textrm{g}$/kg을 mouse에 2일간 투여했을 때 체중, 정자수와 정자활력, 정소중량, 장기중량에 미치는 영향을 조사하였다. 1. Dioxin 10, 20 $\mu\textrm{g}$/kg 투여군의 체중은 30.6$\pm$2.9~40.7$\pm$3.9g, 30.8$\pm$4.1~39.5$\pm$3.1g이었으며, 30, 40 $\mu\textrm{g}$/kg 투여군의 체중은 31.0$\pm$3.5~39.0$\pm$3.5g, 30.6$\pm$3.4~38.3$\pm$4.0g 으로서 대조군의 30.6$\pm$2.8~44.5$\pm$3.1g에 비해 약간 낮은 치를 나타냈으며 dioxin 투여량이 증가할수록 감소하는 경향을 나타냈다(p.0.05). 2. Dioxin 10~40 $\mu\textrm{g}$/kg을 각각 2일간 투여했을때 WBC수는 대조군에 비해 현저한 증가치를 나타냈고 RBC수는 대조군에 비해 다소 증가되었으나 유의한 변화는 인정되지 않았으며, Hb량과 PCV치 및 PLT수는 대조군에 비해 크게 증가된 경향을 나타냈다. 3. Dioxin 10, 20$\mu\textrm{g}$/kg을 투여군의 정자수는 112.5 $\pm$ 3.7 ~ 119.4 $\pm$4.2 $\times$ $10^{6}$$m\ell$, 103.9 $\pm$ 3.8 ~ 110.2 $\pm$ 3.6 $\times$ $10^{6}$$m\ell$이었으며, 30, 40 $\mu\textrm{g}$/kg 투여군의 정자수는 97.5 $\pm$ 3.4 ~105.7 $\pm$ 4.4 $\times$ $10^{6}$$m\ell$, 87.2 $\pm$ 3.7 ~ 98.5 $\pm$ 3.8 $\times$ $10^{6}$$m\ell$로서 대조군의 119.0 $\pm$ 4.3 ~ 120.7 $\pm$ 4.8 $\times$ $10^{6}$$m\ell$에 비해 현저히 감소된 정자수를 나타냈다(p<0.05). 4. 정자활력은 10, 20, 30, 40 $\mu\textrm{g}$/kg을 각각 2일간 투여했을 때 69.4 $\pm$ 3.0 ~ 86.6 $\pm$ 4.7%로서 대조군의 93.0 $\pm$ 3.6 ~ 94.7 $\pm$ 4.2%에 비해 정자의 활력이 현저히 감소되었다(p<0.05). 5. Dioxin 10, 20, 30, 40 $\mu\textrm{g}$/kg을 각각 2일간 투여했을 때 정소중량은 대조군에 비해 약간 감소된 경향을 나타냈다. 6. Dioxin 10, 20, 30, 40 $\mu\textrm{g}$/kg을 각각 2일간 투여했을 때 신장, 비장 및 간의 중량은 정상대조군과 비교할 때 약간의 증가를 나타냈다.
