Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Optimization of Mass cultivation Media for the Production of Biomass and Natural Colourants from Two Marine Cyanobacteria by a Mathematical Design of Experiments

  • Sekar, S. (National Faculty For Marine Cyanobacteria, Bharathidasan University, India) ;
  • Priya, S.Sri Lavanya (National Faculty For Marine Cyanobacteria, Bharathidasan University, India) ;
  • Roy, P.Wesley (National Faculty For Marine Cyanobacteria, Bharathidasan University, India)
  • 발행 : 2000.09.01
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초록

Optimization of chemicals in the large scale sea water medium and inoculum for biomass and natural colourants production in the marine cyanobacteria, Phomidium tenue BDU 46241 (phycoerythrin producer) and P.valderianum BDU 30501 (phycocyanin producer) was carried out by experiments in L8 orthogonal array. Mathematical analysis revealed the significance of these factors. The factor(s) that critically control the yield varied with the organism and the end-product further, the desirable level of these factors between the normal and a higher level tested was identified and improved media were evolved. In both cyanobacteria, higher level of $K_2$$HPO_4$, $NaNO_3$ and inoculum with normal level of ferric ammonium citrate was found to be desirable for biomass production and additionally, higher level of $MgSO_4$ for pigment production. The level of other factors varied with the organism and the end-product. Confirmation experiments showed that the clues obtained based on mathematical experimentation are valid. In P.tenue, the medium optimized for biomass production increased the yield of biomass by 495% and the medium optimized for phycoerythrin production increased the yield of biomass by 408% with 30% increase in phycoerythrin content of the biomass. Similarly in P.valderianum, the medium optimized for biomass production increased the yield of biomass by 224% and the medium optimized for phycocyanin production increased the yield of biomass by 143% with 44% increase in phycocyanin content of the biomass.

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참고문헌

  1. Microalgae: Biotechnology and Microbiology Becker M
  2. Biol Chem v.378 One century of protein crystallography: The Phycobiliproteins Betz M
  3. Algal Biotechnology in the Asia-Pacific Region Products from algae Borowitzka MA;Moi PS(ed.);Kun LY(ed.);Borowitzka MA(ed.);Whitton BA(eds)
  4. Algal Biotechnology in the Asia-Pacific Region Commercial pigment production from algae. Borowitzka LJ;Moi PS(ed.);Kun LY(ed.);Borowitzka MA(ed.);Whitton BA(eds)
  5. Arch Mirobiol v.125 C-Phycocyanin as a storage protein in the blue-green alga Spirulina platensis Boussiba S;Richmond AE
  6. Trends Biochem Sci v.9 Phycoflour probes Glazer AN;Stryer L
  7. J Immunol Methods v.92 The use of phycobiliproteins as fluorescent labels in immunoassay Kronick MN
  8. US Patent 4400400 Acid soluble blue colorant for food products Langston MK;Maing I
  9. J Appl Phycol v.7 Nitrogen-fixing cyanobacteria as source of phycobiliprotein pigments: Composition and growth performance of ten filamentous heterocystous strains Moreno J;Rodriquez M;Vargas A;Rivas J;Guerrero MG
  10. Photosynthesis Research v.11 Large scale preparation of pure phycobiliproteins Padgett MP;Krogman DW
  11. Algal Biotechnology v.7 Large scale microbial culture and applications Richmond A;Round FE(ed.);Chapman DJ(ed.)
  12. J Gen Microbiol v.111 Generic assignments, strain histories and properties of pure cultures of cyanobacteria Rippka P;Deruelles J;Waterbury JB;Herdman M;Stanier RY
  13. Appl Environ Microbiol v.55 Nitrogen fixing cyanobacteria with a high phycoerythrin content Rodriguez H;Rivas J;Guerrero MG;Losada M
  14. Taguchi Techniques for Quality Engineering Ross PJ
  15. Cyanobacterial Biotechnology A method of mass cultivation of the marine cyanobacterium Phormidium valderianum BDU 30501 for the production of blue natural colourant 'Phycocyanin' Seker S;Subramanian G;Subramanian G(ed.);Kaushik BD(ed.);Venkataraman GS(eds)
  16. Manual of Industrial Microbiology and Biotechnology Experimental design for improvement of fermentations Strobel RJ;Sullivan GR;Demain AL(ed.);Davies JE(eds)
  17. Appl Microbiol Biotechnol v.31 Effect of nutritional factors on the culture of Nostoc sp. as a source of phycobiliproteins Silva H;Cortinas TI;Ertola RJ
  18. Biochemistry Stryer L.
  19. CyanobacterialBiotechnology Production of useful materials by marine microalgae Takeyama H;Matsunaga T;Subramanian G(ed.);Kaushik BD(ed.);Venkataraman GS(eds)
  20. Appl Microbiol Biotechnol v.24 Outdoor mass culture of Spirulina maxima in sea-water Tredici MR;Papuzzo T;Tomaselli L
  21. Phykos v.28 Spirulina: state of the art and emerging prospects Venkataraman LV