Rapid Assesment of Microspore Development Stage in Pepper Using DAPI and Ferric chloride

  • Kim, Moon-Za (Department of Biological Science, Mokwon University) ;
  • Jang, In-Chang (Department of Biological Science, Mokwon University)
  • 발행 : 2000.09.01

초록

Clear visualization of pepper (Capsicum annuum L.) microspore nuclei with common stains such as acetocarmine or propionocarmine is difficult, hindering cytological analysis. The DAPI stain after the addition of ferric chloride solution to fixative resulted in clear visualization of nuclei. For clear visualization of nuclei and slight fluorescence of microspore wall, addition of 40-60 ${mu}ell$ of ferric chloride solution to the 1 $m\ell$ fixative was identified as most effective. At all stages of gametophytic development, the nuclei can be distinctly visualized. Starch granules does not intefere with the fluorochrome, and so the vegetative and generative nuclei were cleary visible in binucleate pollens. With its rapidity and reliability, this technique represents an efficient tool for routine staging or investigation of the nuclear status of the microspore during culture.

키워드

참고문헌

  1. Biol Bul v.50 The iron-acetocarmine method of fixing and staining chromosomes Belling J
  2. The embryology of angiosperms Male gametophyte-de-velopment Bhojwani SS;Bhatnagar SP
  3. Stain Technol v.60 Applications of fluorochromes to pollen biology. 1. Mithramycin and 4',6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA. Coleman AW;Goff LJ
  4. Protoplasma v.147 Development of microspore in vivo and in vitro in Brassica napus L. Fan Z;Armstrong C;Keller WA
  5. Stain Technol v.60 A new technique for retarding fading of fluorescence: DPX-BME Franklin AL;Filion WG
  6. Protoplasma v.78 Haploid Capsicum through experimental androgenesis George L;Narayanaswamy S
  7. Theor Appl Genet v.74 Improved rate of callus in duction from rice anther culture following microscopic staging of microspores in iron alum-haematoxylin Gupta HS;Borthakur DN
  8. Secual plant reproduction Cytological techniques to assess pollen quality Heslop-Harrison JS;Cresti M(ed.);Tiezzi A(eds)
  9. Stain Technol v.60 Applications of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst 33258 in conjunction with the callose-specific anilin blue fluorochrome Hough T;Bernhardt P;Knox RB;Williams EG
  10. Korea J Plant Tissue Culture v.19 Development of microspores during anther culture of Brassica napus L. Kim MZ
  11. Korea J Plant Tissue Culture v.12 Basic studies on the induction of microspore-originated calluses or embryos in the anther culture of Capsicum annuum L. Kim MZ;Kim YR
  12. Can J Bot v.66 Cytological aspects of isolated microspore culture of Brassica napus Kott LS;Polsoni L;Beversdorf WD
  13. Stain Technol v.27 A controllable carmine technique for plants with small chromosomes Marks GE
  14. Stain Technol v.299 An acetocarmine technique for Curcurbita McGoldrick PT;Bohn GW;Whitacker TW
  15. Stain Technol v.60 A comparative sthdy of DAPI, DIPI, and Hoechst 33258 as chromosomal DNA stains Otto F;Tsou KC
  16. Theor Appl Genet v.73 Anther culture of maize and the vizualization of embryogenic microspores by fluorescent microscopy Pace GM;Reed JN;Ho LC;Fahey JW
  17. Physiol Plant v.74 Identification of potentially embryogenic microspores in Brassica napus Pechan PM;Keller WA
  18. Vitro Cell Dev Biol v.33 Development of asparagus microspores in vivo and in vitro is influenced by gametogenic stage and cold treatment Peng M;Ziauddin A;Wolyn DJ
  19. Plant Cell Reports v.7 Microspore development in cultured maize anthers Pescitelli SM;Petolino JF
  20. vitro haploid production in higher plants v.3 Anther and microspore culture in Capsicum Regner F;Jain SM(ed.);Sopory SK(ed.);Veilleux RE(ed.)
  21. Plant Physiol v.77 Initiation of post-meiotic B galactosidase synthesis during microsporogenesis in oil seed rape Singh MB;O'Neill P;Knox RB
  22. Stain Technol v.62 Rapid assessment of microspore and pollen develoopment stage in wheat and maize using DAPI and membrane permeabilization Vergne P;Delvallee I;Dumas C
  23. Plant Cell Tissue Organ Culture v.24 Effect of microspore stage and media on anther culture of peanut (Arachis hypogaea L.) Willcox MC;Reed SM;Burns JA;Wynne JC
  24. HortScience v.32 Improved nuclear staining of asparagus microspores for cytological analysis Ziauddin A;Peng M;Wolyn DJ