Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

The Factors on Somatic Embryogenesis of Soybean [Glycine max. (L.) Merrill]

  • Kim, Kyong-Ho (National Honam Agricultural Experiment Station R.D.A) ;
  • Kim, Hag-Sin (National Honam Agricultural Experiment Station R.D.A) ;
  • Oh, Young-Jin (National Honam Agricultural Experiment Station R.D.A) ;
  • Suh, Sug-Kee (National Honam Agricultural Experiment Station R.D.A) ;
  • Kim, Tae-Soo (National Honam Agricultural Experiment Station R.D.A) ;
  • Park, Ho-Kee (National Honam Agricultural Experiment Station R.D.A) ;
  • Park, Moon-Soo (National Honam Agricultural Experiment Station R.D.A) ;
  • Kim, Seok-Dong (National Honam Agricultural Experiment Station R.D.A) ;
  • Yeo, Up-Dong (Faculty of Biological Science, College of Natural Sciences, Chonbuk National University)
  • Published : 2000.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as material part and size of immature seed, 2,4-D, pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up to 22.9%) of somatic embryogenesis was obtained from the immature cotyledon, following embryonic axis and the immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25.4%) of embryogenesis was obtained from 3-4 mm length of the seed (after 25 days of flowering). The optimum concentration of 2,4-D for embryogenesis was 10 mg/L. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tips from the germinating somatic embryos, they were cultured on MS medium containing 2 mg/L indole-3-butyyic acid (IBA) and 1 mg/L 6-benzyladenine (BA). After then somatic embryos with one and three cotyledons were transferred to the growth regulator free medium. The medium exhibited the higher rate (ca. 50%) of development than the multiplication medium.

Keywords

References

  1. Euphytica v.80 New aspects of soybean somatic embryogenesis Anna NO;Waclaw O
  2. Plant Sci v.93 Genotype-specific optimization of plant regeneration from somatic embryos of soybean Bailey MA;Boerma HR;Parrott WA
  3. Planta v.167 Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis Barwale UB;Kerns HR;Widholm JM
  4. Plant Physiol v.89 Maturation of soybean somatic embryos and the transition to plantlet growth Buchheim JA;Colburn SM;Ranch JP
  5. Korean J Plant Tissue Culture v.21 Somatic embryogenesis in immature zygotic embryo cultures of Korean soybean (Glycine max L.) cultivars and effect of 2,4-D on somatic embryo morphology Choi PS;Soh WY;Cho DY;Liu JR
  6. Vitro Cell Dev Biol v.27 Transformation of soybean via particle bombardment of embryogenic suspension culture tissue Finer JJ;McMullen MD
  7. Planat Cell Tissue Organ Cult v.15 Development of an embryogenic suspension culture of [Glycine max. (L.) Merrill]. Finer JJ;Nagasawa A
  8. Plant Cell Reports v.5 Somatic embryogenesis and plant regeneration from embryonic callus of soybean Ghazi TD;Cheema HV;Nabors MW
  9. Biol/Technology v.6 Production of transgenic soybean plants using Agrobacteriummediated DNA transfer Hinchee MA;Connor-Ward DV;Newell CA
  10. Vitro Cell Dev Biol v.31 Effects of common components on hardness of culture media prepared with $Gelite^{TM}$ Huang LC;Kohashi C;Vangundy R
  11. Plant Physiol v.136 Plant regeneration in vitro from primary leaf nodes of soybean (Glycine max) seedlings Kim JH;LaMotte GE;Hack E
  12. Plant Mol Biol Reports v.3 A Procedure for plant regeneration from immature cotyledon tissue of soybean Lazzeri PA;Hildebrand DF;Collins GB
  13. Plant Cell Reports v.4 Somatic embryogenesis and plantlet regeneration in the soybean Glycine max Li BJ;Langridge WHR;Szalay AA
  14. Merr. Plant Cell Reports v.4 Induction of somatic embryos in cotyledonary tissue of soybean Glycine maxL. Lippmann B;Lippmann G
  15. Vitro Cell Dev Biol v.28 Somatic embryogenesis in soybean via somatic embryo cycling propagation Liu W;Moore PJ;Collins GB
  16. Bio/Technology v.6 Stable transformation of soybean (Glycine max) by particle acceleration McCabe DE;Swain WF;Mertinell BJ
  17. Physiol Plant v.5 A revised medium for rapid growth and bioassay with tobacco cultures Murashige T;Skoog F
  18. Introductory Plant Physiology. Second Edition Noggle GR;Fritz GJ
  19. Vitro Cell Dev Biol v.30 Recovery and evaluation of soybean plants transgenic for a Bacillus thuringiensis var. Kurstaki insecticidal gene Parrot WA;All JN;Adang MJ
  20. Vitro Cell Dev Biol-Plant v.33 Somatic embryogenesis from cultured epicotyls and primary leaves of soybean [Glycine max. (L) Merrill] Rajasekaran K;Pellow JW
  21. Vitro v.21 Plant regeneration from embryo-derived tissue cultures of soybean Ranch JP;Oglesby L;Zielinski AC
  22. Vitro Cell Dev Biol-Plant v.33 Effect of explant orientation, pH, solidifying agent and wounding on initiation of soybean somatic embryos Santarem ER;Pelissier B;Finer JJ
  23. Plant Cell Reports v.12 Stable transformation via particle bombardment in two different soybean regeneration systems Sato S;Newell C;Kolacz K
  24. Korean J Breeding v.27 Somatic embryogenesis and plant regeneration from in vitro cultured immature cotyledons of soybean Sohn JK;Bae JS
  25. CR Seances Acad Sci Ser v.3 vitro somatic embryogenesis and plant regeneration in three precocious varieties of soybean Tetu T;Sangwan-Norreel B;Sangwan R
  26. Transformation and regeneration via cotyledonary nodes Townsend JA;Thomas LA
  27. Plant Cell Reports v.5 Plant regeneration by organogenesis in Glycine max Wright MS;Koehler SM;Hinchee MA
  28. Vitro Cell Dev Biol v.27 A simple method for the recovery of multiplefertile plants from individual somatic embryos of soybean [Glycine max. (L.) Merrill]. Wright MS;Launis KL;Novitzky R
  29. Plant Cell Reports v.6 Regeneration of soybean [Glycine max. (L.) Merrill] from cultured primary leaf tissue Wright MS;Ward DV;Hinchee MA
  30. J Plant Biol v.42 Turnover of cell-wall polysaccharides during somatic embryogenesis and development of celery (Apium graveolens L.) Yeo UD;Han JY;Choi YE