Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Structure and Function of NtCDPK1, a Calcium-dependent Protein Kinase in Tobccco

  • Yoon, Gyeong-Mee (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sang-Sook (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Pai, Hyun-Sook (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2000.05.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

We have isolated a cDNA encoding a calcium-dependent protein kinase (CDPK) in Nicotiana tabacum, which was designated NtCDPK1. Accumulation of the NtCDPK1 mRNA was stimulated by various stimuli, including phytohormones, CaCl$_2$ wounding, fungal elicitors, chitin and methyl jasmonate. The NtCDPK1 gene encodes a functional Ser/Thr protein kinase of which phosphorylation activity is strongly induced by calcium. By analyzing expression of the NtCDPK1-GFP fusion protein and by immunoblotting with antibody which reacts with NtCDPK1, we found that NtCDPK1 is localized in membrane and nucleus in plant cells. Silencing expression of the NtCDPK1 transgene resulted in marked decrease of lateral root development in the transgenic tobacco plants. Yeast two hybrid screening using NtCDPK1 as a bait identified a tobacco homologue of proteasome regulatory subunit 21D7, designated Nt21D7. The 21D7 mRNA has been shown to be predominantly expressed in proliferating tissues in the cell cycledependent manner in carrot. The recombinant NtCDPK1 protein associated with Nt21D7 in vitro, and could phosphorylate the Nt21D7 protein in vitro in the presence of calcium, suggesting that Nt21D7 protein is a natural substrate of NtCDPK1 in tobacco. These results suggest that NtCDPK1 may regulate tell proliferation processes, such as lateral root formation, by regulating specificity and/or activity of proteasome-mediated protein degradation pathway.

키워드

참고문헌

  1. Plant Mol Biol v.30 Calcium-dependent protein kinase gene expression in responase to physiol and chemical stimuli in mungbean(Vigna radiata) Botella JR;Arteca JM;Somodevilla M;Arteca RN
  2. Plant Mol Biol v.27 Molecular cloning of two novel rice cDNA sequences encoding putative calcium-dependent protein kinases Breviario D;Morello L;Giani S
  3. Annu Rev Plant Physiol Plant Mol v.46 Calcium regulation in plant cells and its role in signaling Bush DS
  4. Proc Natl Acad Sci USA v.91 CLoning and characterization of maize pollen-specific calcium-dependent calmodulin-independent protein kinase Estruch JJ;Kadwell S;Merlin E;Crossland L
  5. Science v.252 A calcium-dependent protein kinase with a regulatory domain similar to camodulin Harper JF;Sussman MR;Schaller GE;Putham-Evans C;Charbonneau H;Harmon AC
  6. Plant Mol Biol v.30 Expression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thaliana Hong Y;Takano M;Liu C-M;Gasch A;Chye M-L;Chua N-H
  7. Plant Physiol v.100 Tonoplast-bound protein kinase phosphorylates tonoplast intrinsic protein Johnson KD;Chrispeels MJ
  8. Annu Rev Plant Physiol Plant Mol Biol v.43 Calcium-modulated proteins: Targets of intracellular calcium signals in higher plants Robert DM;Harmon AC
  9. Science v.274 $Ca^++$-dependent protein kinases and stress signal transduction in plants Sheen J
  10. Plant Mol Biol v.17 Osolation and sequence analysis of a cDNA clone for a carrot calcium-dependent protein kinases and to calmodulin Suen K-L;Choi JH
  11. Mol Gen Genet v.244 Two genes that encode $Ca^++$-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana Urao T;Katagiri T;Mizoguchi T;Yamaguchi-Shinozaki K;Hayashida N;Shinozaki K
  12. Plant Mol Biol v.39 Chatact erization of NtCDPK1, a dalcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein Yoon GM;Cho HS;Ha HJ;Liu JR;Lee H-S