Linkage Map Construction and Molecular Genetic Approach in Capsicum spp.

  • Kim, Byung-Dong (Division of Plant Science, and the Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
  • 발행 : 2000.09.01

초록

We have constructed a molecular linkage map of chili pepper (Capsicum spp.) in an interspecific (C. annuum cv. TF68 x C. chinense cv. Habanero) F$_2$ population of 107 plants with 150 RFLP and 430 AFLP markers. The resulting linkage map consists of 11 large (206-60.3 cM) and 5 small (32.6- 10.3 cM) linkage groups cover-ing 1,320 cM with an average map distance between framework markers of 7.5 cM. Most (80%) of the RFLP markers were pepper-derived clones and these markers were evenly distributed across the genome. By using 30 primer combinations, 444 AFLP markers were generated in the F$_2$population. The majority of the AFLP markers clustered in each linkage group, although PstI/MseI markers were more evenly distributed than Eco RI/MseI markers within the linkage groups. Genes for biosynthesis of carotenoids and capsaicinoids were mapped on our linkage map. This map will provide the basis of studying secondary metabolites in pepper.

키워드

참고문헌

  1. Theor Appl Genet v.94 QTLs for a component of partial resistance to cucumber mosaic virus in pepper: restriction of virus installation in host-cells Caranta C;Palloix A;Lefebvre V;Daubeze A-M
  2. Capsicum and Eggplant Newsletter v.13 Updated Capsicum gene list Daskalov S;Poulos JM
  3. Theor Appl Genet. Candidate gene approach identified phytoene synthase as the locus for mature fruit color in red pepper (Capsicum spp.) Huh JH;Kang BC;Nahm SH;Kim S;Ha KS Lee MH;Kim BD
  4. L. J Hered v.76 Inheritance of mature fruit color in Capsicum annuum Hurtado-Hernandez H;Smith PG
  5. Theor Appl Genet. An Interspecific (Capsicum annuum × C. chinense) F₂Linkage Map in Pepper Using RFLP and AFLPM Markers Kang BC;Nahm SH;Huh JH;Yoo HS;Lee MH;Kim BD
  6. J Plant Biol v.40 Construction of a molecular linkage map and development of a molecular breeding technique Kim BD;Kang BC;Nahm SH;Kim BS;Kim NS;Lee MH;Ha KS
  7. J Kor Soc Hort Sci v.38 Analysis of chemical constituents in fruits of red pepper (Capsicum annuum L.cv. Bugang) Kim SH;Kim YH;Lee ZW;Kim Bd;Ha KS
  8. Acta Bot v.6 Die Genetischen Typen der Carotinoid-systeme der Paprikafrucht Kormos J;Kormos K
  9. Plant Mol Biol v.36 The capsanthin-capsorubin synthase gene: a candidate gene for the ylocus controlling the red fruit colour in pepper Lefebvre V;Kuntz M;Camara B;Palloix A
  10. Theor Appl Genet v.93 Both epistatic and additive effects of QTLs are involved in polygenic induced resistance to disease: a case study, the interaction pepper - Phytophthora capsaici Leonian Lefebvre V;Palloix A
  11. Genetics v.152 Genome mapping in capsicum and evolution of genome structure in Solanaceae Livingstone KD;Lackney VK;Blauth JR;Van Wijk R;Jahn MK
  12. Plant J v.3 PCR amplified microsatellites as markers in plant genetics Morgante M;Olivieri AM
  13. Molecular Plant Microbe Interactions v.11 Genetic mapping of the pur1 locus in Capsicum and evidence that distinct potyvirus resistance loci control responses that differ at the cellular and whole plant level Murphy JF;Blauth JR;Livingstone KD;Lackney VK;Jahn MK
  14. J Kor Soc Hort Sci v.38 no.6 Selection of parental lines for hot pepper mapping population usin gRFLP and AFLP analysis Nahm SH;Yu JW;Kang BC;Kim BD
  15. Genome v.36 Construction of a molecular linkage map of pepper and a comparison of synteny with tomato Prince, JP;Pochard E;Tanksley SD
  16. Genome v.36 Construction of a molecular linkage map of pepper and a comparison of synteny with tomato Prince, JP;Pochard E;Tanksley SD