Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Genomic and Transgenic Approaches to Modified Plants: Disease Resistance in the Brassica as a Model System.

  • Ekuere, Usukuma (Department of Biological Sciences, University of Alberta, Edmonton Alberta) ;
  • Good, Allen G. (Department of Biological Sciences, University of Alberta, Edmonton Alberta) ;
  • Mayerhofer, Reinhold (Department of Biological Sciences, University of Alberta, Edmonton Alberta)
  • Published : 2000.07.01
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Abstract

Molecular genetic techniques can now be applied to the development of advanced plant genotypes, either through genetic transformation or genomic approaches which allow researchers to transfer specific traits using molecular markers. In this paper, we discuss the use of these techniques towards understanding the genetics of blackleg resistance in Brassica. In a comparative mapping study between Arabidopsis thaliana and Brassica napus, 6 R-ESTs, 7 B. napus RFLP markers and a B. napus EST were located in a collinear region of N7 (B. napus) and chromosome 1 (A. thaliana). One of the A. thaliana R-ESTs and 4 of the B. napus RFLPs co-segregated and mapped to the LmRl locus for blackleg resistance. Introgression of blackleg resistance from wild relatives is also investigated with the possibility of accelerating the introgression process via marker assisted selection.

Keywords

References

  1. Mol Plant-Microbe Interact v.11 Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana Aarts MGM;Hekkert BTL;Holub EB;Beynon JL;Stiekema WJ;Pereira A
  2. Plant pathology, ($4^{th}$ ed.) Agios GN
  3. Plant Dis v.78 A comparison of greenhouse and field screening methods for blackleg resistance in DH lines of Brassica napus Bansal VK;Kharbanda PD;Stringham GR;Thiagarajah MR Terwari JP
  4. Plant Cell v.8 Disease resistance in genes: function meets structure Bent AF
  5. Plant J v.12 Map position of 47 Arabidopsis sequences with sequence similarity to disease resistance genes Botella MA;Coleman MJ;Hughes DE;Nishimura MT;Jones JDG;Somerville SC
  6. Genome v.41 Collinearity between a 30-centimorgan segment of Arabidopsis thaliana chromosome 4 and duplicated regions within the Brassica napus genome Cavell AC;Lydiate DJ;Parkin IAP;Dean C;Trick M
  7. Theor Appl. Genet v.91 RFLP mapping of resistance to the blackleg disease [causal agent, Leptosphaeria maculans (Desm.) Ces. et de Not.] in canola (Brassica napus L.) Dion Y;Gugel Rk;Rakow GFW;S$\'{e}$guin-Swartz G;Landry BS
  8. Phytopathology v.85 Mapping loci controlling brassica napus resistance to Leptosphaeria maculans under different screening conditions Ferreira ME;Rimmer SR;Williams PH;Osborn TC
  9. Theor Appl Genet v.94 The genetics of stamenoid petal production in oilseed rape (Brassica napus) and equivalent variation in Arabidopsis thaliana Fray MJ;Puangsomlee P;Goodrich J;Coupland G;Evans EJ;Arthur AE;Lydiate DJ
  10. Can J Plant Pathol v.14 History, occurrence, impact and control of blackleg of rapeseed Gugel RK;Petrie GA
  11. Addvances in Botanical Research v.24 The role of leucine-rich repeat proteins in plant defense Jones DA;Jones JDG
  12. Proc Natl Acad Sci USA v.93 Resistance gene analogs are conserved and clustered in soybean Kanazin V;Marek LF;Shoemaker RC
  13. Trends Genet v.12 A useful weed put to work genetic analysis of disease resistance in Arabidopsis thaliana Kunkel BA
  14. Plant J v.9 Comparative mapping in Arabidopsis and Brassica, fine scale genome collinearity and congruence of genes controlling flowering time Lagercrantz U;Putterill J;Coupland G;Lydiate DJ
  15. Nature Genet v.14 A PCR-Based approach for isolating pathogen resistance genes from potato with potential for wide applications in plants Leister D;Ballvora A;Salamini F;Gebhardt C
  16. Plant J v.4 Recombinant inbred lines for mapping RFLP and phenotypic markers in A. thaliana lister C;Dean C
  17. Genome v.40 Molecular mapping of resistance to Leptosphaeria maculans in Australian cultivars of Brassica napus Mayerhofer R;Bansal VK;Thiagarajah MR;Stringam GR;Good AG
  18. Proc. $10^{th}$ Intermational Rapeseed Congress Mapping and characterization of blackleg resistance gene in Brassica napus Mayerhofer R;Stringam G;Bansal VK;Thiagarajah MR;Parkin IAP;Good AG
  19. Proc Nat Acad Sci USA v.88 Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific regions by using segregating populations Michelmore RW;Paran I;Kesseli RV
  20. Plant Cell v.10 The root knot nematode resistance gene Mi is a member of the leucine zipper, nucleotide binding, leucine-rich family of plant genes Milligan SB;Bodeau J;Yaghoobi J;Kaloshian I;Zabel P;Williamson V
  21. Theor Appl Genet v.89 Somatic hybrids with substitution type genomic configuration TCBB for the transfer of nuclear and organelle genes from Brassica tournefortii TT to allotetraploid oilseed crop B. carinata BBCC Mukhopadhyay A;Arumugam N;Pradhan AK;Murthy HN;Yadav BS;Sodhi YS;Pental D
  22. Theor Appl Genet v.92 transfer of Brassica tournefortii (TT) genes to allotetraploid oilseed Brassica species (B. juncea AABB, B napus, AACC, B. Carinata BBCC): homeologous pairing is more pronounced in the three genome hybrids (TACC, TBAA, TCAA, TCBB) as compared to allodiploids (TA, TB, TC) Nagpal R;Raina SN;Sodhi YS;Mukhopadhyay A;Arumugam N;Pradhan AK;Pental D
  23. Genetics v.146 Comparison of flowering time genes in Brassica rapa, B. napus and Arabidopsis thaliana Osborn TC;Kole C;Parkin IAP;Sharpe AG;Kuiper M;Lydiate DJ;Trick M
  24. Plant Breeding v.100 Effective transfer of cytoplasmic male sterillity from radish (Raphinus sativa) to rape Brassica napus L. Paulman W;Robbelen G
  25. Genome v.38 Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape) Parkin IAP;Sharpe AG;Keith DJ;Lydiate DJ
  26. Eur J Plant Path v.104 Reaction of Brassica juncea (Indian mustard) lines to Australian isolates of Leptosphaeria maculans under glasshouse and field conditions Purwantara A;Salisbury PA;Burton WA;Howlett BJ
  27. Theor Appl Genet v.75 Interspecific hybrids between Brassica napus L. and B. oleracea L. developed by embryo culture Quazi HM
  28. Proc Natl Acad Sci USA v.83 Meiosis in sesquidiploid hybrids of Lycopersicum esculentum and Solanum lycopersicoides Rick CM;DeVema JW;Chetelat RT;Stevens MA
  29. Proc. $10^{th}$ Intermational Rapeseed Congress Mapping resistance genes in Brassica napus to Leptosphaeria maculans Rimmer SR;Borhan MH;Zhu B;Somers D
  30. Theor Appl Genet v.94 Desaturase multigene families of Brassica napus arose through genome duplication Scheffler JA;Sharpe AG;Schmidt H;Sperling P;Parkin IAP Lydiate DJ;Heinz E
  31. Genome v.38 Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus) Sharpe AG;Parkin IAP;Keith DJ;Lydiate DJ
  32. Genome v.43 Arabidopsis thaliana: a source of candidate disease-resistance genes for Brassica napus Sillito d;Parkin IAP;Mayerhofer R;Lydiate DJ;Good AG
  33. Genome v.41 a superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum) Spielmeyer W;Robertson M;Collins N;Leister D;Schulze-Lefert P;Seah S;Moullet O;Lagudah ES
  34. $13^{th}$ International Eucarpia Congress Genetic analysis of blackleg (Leptosphaeria maculans) resistance in Brassica napus L. using the doubled haploid method Stringam GR;Bansal VK;Thiagarajah MR;Degenhardt DF;Tewari JP
  35. Theor Appl Genet v.75 Use of molecular markers in breeding for soluble solids content in tomato - a re-examination Tanksley SD;Hewitt J
  36. Genetics v.152 Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis Warren RF;Merritt PM;Holub EB;Innes RW
  37. Can J Plant Path v.14 Biology of Leptoshaeria maculans Williams PH
  38. Proc Natl Acad Sci USA v.93 Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site Yu YG;Buss GR;Saghai Maroof MA