Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Cadmium on Somatic Embryogenesis from Cell Culture of Daucus carota L.

당근(Daucus carota L.)의 현탁배양을 통한 체세포배 발생에 미치는 카드뮴의 영향

  • 조덕이 (우석대학교 이공대학 생물학과) ;
  • 신은경 (전북대학교 자연과학대학 생물과학부) ;
  • 소웅영 (전북대학교 자연과학대학 생물과학부)
  • Published : 2000.05.01
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Abstract

This study was carried out to elucidate the effect of cadmium on somatic embryogenesis and plant regeneration from cultured cells of Daucus carota L. Embryogenic calli were induced from cotyledon explants of carrot seedlings cultured on MS solid medium supplemente with 1 mg/L 2,4-D Embryogenic cells proliferated on medium supplemented with 1 mg/L 2,4-D were also cultured in liquid MS medium containing various concentrations (50, 100, 200, 500, 1000 $\mu$M) of cadmium for one week and then transferred to MS basal medium. Somatic embryogenesis occurred in suspension culture treated with 50 $\mu$M and 100 $\mu$M cadmium or untreated with cadmium. When cadmium was treated in suspension culture, production of two and four cotyledonary somatic embryos was reduced, but that of three cotyledonary somatic embryo was increased. Two cotyledonary embryos showed higher regeneration frequency than abnormal somatic embryo with one, three and four cotyledon. Regardless of cotyledonary variation, germination frequency of somatic embryos treated with cadmium was decreased in compared with that of embryos in basal medium.

당근 유식물체의 자엽을 절편으로 유도된 배형성능 세포를 여러가지 농도의 카드뮴 (0, 50. 100, 200. 500, 1000 $\mu$M)이 첨가된 MS 액체배지에 배양한 다음 MS 기본배지에 옮겨서 체세포배 발생을 유도했고 한편 카드뮴 처리구와 카드뮴이 처리되지 않은 대조구에서 체세포배를 얻었다. 현탁배양에서 정상적인 2개의 자엽을 갖는 체세포배 발생은 카드뮴 첨가 배지에서 감소하였으나 비정상적인 3개의 자엽을 갖는 체세포배가 많이 관찰되었다. 또한 비정상적인 1개, 3개 및 4개의 자엽을 갖는 체세포배보다 정상적인 2개의 자엽을 갖는 체세포배의 식물체 재생률이 가장 높았다. 카드뮴 처리구에서 발생된 체세포배의 발아율은 자엽수에 관계없이 대조구에서 발생된 체세포배의 발아율에 비해 감소하였다.

Keywords

References

  1. Tissue Culture in Culture in Foresty and Agriculture Patterns of developement in culture Ammirato PV;R.R. Henke(eds.);K.W. hunghes(eds.);M.P. Constantin(eds.);A. Hollanender(eds.)
  2. Plant Tissue and Cell Culture Organizeational events during somatic embryogenesis Ammirato PV;CE Green(et al. eds.)
  3. Bot Gaz v.132 Some effects of the environment on the development of embryos from cultured free cells. Ammirato PV;Steward FC
  4. Plant Cell Rep v.3 Selection and characterization of cadmium-resisiant suspension cultures of the wild tomato. Lycopersicon peruvianum Bennetzen JL;Adams TL
  5. Plant Physiol v.89 Maturation of soybean somatic embryos and the transition to plantlet growth Buchheim JA;Colburn SM;Ranch JP
  6. Kor J Plant Tiss Cult v.25 Anomalous structure of somatic embryos developed from leaf explant cultures of Angelica gigas Nakai Cho DY;Lee EK;Soh WY
  7. M. Kor J Plant Tiss Cult v.27 Cotyledon structure and germinability of somatic embryos formed from inflorescence explants of Cnidium officinale Cho DY;Lee EK;Soh WY
  8. Kor J Plant Tiss Cult v.22 Morphological observation of somatic embryogenesis in leaf explant cultures of Bupleurum falcatum L. Cho DY;Soh WY
  9. Kor J Plant Tiss Cult v.21 High frequency Somatic embryogenesis and plant regeneration in seeding explant cultures of melon (Cucumis melo L.) Choi PS;Soh WY;Cho DY;Liu JR
  10. Kor J Plant Tiss Cult v.21 Somatic embryogenesis in immature zygotic embryo culturesof korean soybean (Glycine max L.) cultivars and effect of 2,4-Dichlorophenoxy acetic acid on somatic embryo morphology Choi PS;Soh WY;Cho DY;Liu JR
  11. Can J Plant Sci v.74 Effect of zinc on cadmium concentration in the tissue of durum wheat Choudhary M;Bailey LD;Grant CA
  12. J Amer Soc Hort Sci v.118 High-frequency somatic embryogenesis from quiescent seed cotyledons of Cucumis melo cultivars Gray DJ;McColley DW;Compton ME
  13. Plant Sci v.52 Selection and characterization of cadmium tolerant cells in tomato Huang B;Hatch E;Goldsbrough PB
  14. Plant Cell Tiss Org Cult v.33 Arrest of somatic embryo development in grapevine : Histological characterization and the effect of ABA, BAP and Zeatin in stimulating plantlet development Isabelle GT;Mauro MC;Sossountzov L;Miginiac E;Deloire A
  15. Environmental Injury to Plants Mechanisms of trace metal tolerance in plants Jackson PJ;Unkefer CJ;Doolen JA;Robinson NJ;F Katterman(ed.)
  16. Proc Natl Acad Sci USA v.84 Poly(r-glutamyl cysteinyl) glycines:Its role in cadmium resistance in plant cells Jackson PJ;Unkefer CJ;Doolen JA;Watt K;Robinson NJ
  17. Amer J Bot v.76 Histochemistry and ultrastructure of rice (Oryza sativa) zygotic embryogenesis Jones TD;Rost TL
  18. Plant Tiss Cult Lett v.7 Effects of sucrose concentration on morphology of somatic embryos from immature soymean cotyledons Kageyama K;Komatsuda T;Nakajima K
  19. In Vrtro Cell Dev Biol v.25 Stress induced somatic embryogenesis in carrot and its application tod synthetic seed production Kamada H;Kobayashi K;Kiyosue T;Harada H
  20. Acta Horticult v.319 Induction of carrot somatic embryogenesis by high sucrose concentration without changing culture medium Kurata K;Iwabuchi;Futaya Y
  21. J Plant Biol v.40 Effect of humidity on somatic embryogenesis in cotyledon explant culture of carrot Lee EK;Cho DY;Soh WY
  22. Kor J Limnol v.23 Cadmium detoxification mechanism of Cd-resistant Bacillus sp. Isolated from industrial sewage Lee KS;Kang WS;Kim YH;Kim EA;Kim KH;Choi YK
  23. Thunb Kor J Plant Tiss Cult v.20 Somatic Embryogenesis and structural aberrancy of embryos in tissue cultures of Aralia cordata Lee KS;Soh WY
  24. Thunb. Kor J Plant Tiss Cult v.20 Effects of cytokinins on the number of cotyledons of somatic embryos from cultured cells of Aralia cordata Lee KS;Soh WY
  25. Physiol Plant v.15 A rivised medium for rapid growth and bioassays with tobacco tissue Murashige T;Skoog F
  26. J Exp bot v.30 Embryos and plantlets from cultured anthers of hybrid grapevines Rajasekaran K;Mullins MG
  27. Plant Cell Rep v.3 Cadmium uptake and its effects on growth of tobacco cell suspension cultures Reese RN;Roberts LW
  28. Phytomorphology v.44 Anatomical changes in root and stem of wheat (Triticum aesstivum L.) in response to different heavy metals Setia RC;Bala R
  29. Progress in plant Cellular and Molecular Biology pH control of carrot somatic embryogenesis. Smith DL;Krikorian AD;Hjj Nijkamp(eds.);LHW Van der plas(eds.);J Van der Plas(eds.);J Van Artrijk(eds.)
  30. Kor J Bot v.39 Multicotyledonary structure of somatic embryos formed from cell cultures of Daucus carota L. Sch WY;Cho DY;Lee EK
  31. Kor J Plant Tiss Cult v.25 Enhanced development and germination of carrot somatic embryos on modified surface of medium Soh WY;Jeon KB;Cho DY
  32. Kor J Plant Tiss Cult v.7 The effects of cadmium and auxin on the adventitious root formation in hypocotyl segments of Phaseolus vulgaris L. Soh WY;Jeon KB;Cho DY
  33. Physiol Plant v.85 Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation Somashekaraiah BV;Padmaja K;Prasad ARK
  34. Amer J Bot v.445 Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended Steward FC;Mapes MO;Mears K
  35. Kor J Bot v.19 Studies on the precipitation of lead ion and the inhibition of plnat growth Sung MW
  36. Plant Science v.120 Enhancing carrot somatic embryos survival during slow dehydration, by encapsulation and control of dehydration Timbert R;Barbotin JN;Thomas D
  37. Ann Rev Biochem v.41 Biochemical effects of mercury cadmium and lead Vallee BL;Ulmer DD
  38. Plant Science v.92 The relationship between somatic embryo morphology and conversion in peaunt (Arachis hypogaea L.) Wetzstein HY;Baker CM
  39. Plant Cell v.12 Novel regulation of heat shock genes during carrot somatic embryo development Zimmerman JL;Apuya N;Darwish KO;Carroll C