Abstract
This study was carried out to identify varietal difference and the inheritance of the ability of plant regeneration in anther culture of rice. The anthers of 33 Japonica, 11 Indica/Japonica, and 3 Indica rice cultivars were culture on $N_6-Y$_1$$ medium with 1 mg/L NAA and 2 mg/L kinetin. The remarkable variability in plant regeneration were observed among the genotypes, ranging from 0.0% to 19.4% Most of Japonica rices were resulted to have better culturability than that of Indica/Japonica and Indica type cultivars. Newly developed Japonica rices, 'Ilmibyeo' and 'Hangnambyeo' showed to have regenerability with the frequency of 19.4% and 18.1% respectively. The segregation mode for callus formation and plant regenerationin anther culture of F$_2$ population of 'Milyang 23/Chucheongbyeo' showed a continuous variation. The variation of plant regeneration frequency in anther culture of F$_2$ plants ranged from 0% to 33.3% with a mean of 6.3% The broad-sense heritability estimated from the ability of plant regeneration was considerably high (82.7%).
벼 약배양에서 식물체 재분화 능력의 품종간 차이와 유전 양식에 대해 연구한 결과는 다음과 같다. ‘일미벼’ 외 46품종의 녹색체 재분화율은 자포니카형이 통일형이나 인디카형보다 높게 나타났고, 동일 품종 생태형간에도 배양 효율은 다양한 변이폭을 나타내었다. 자포니카 품종중에서 ‘일미벼’와 ‘향남벼’는 각각 19.4%와 18.1%의 높은 재분화 능력을 나타내었다. ‘밀양 23호’와 ‘추청벼’가 교배된 F$_2$집단의 녹색체 재분화율은 평균 6.6%로 나타났고 0~33.3%까지 넓은 변이폭을 가지는 연속적인 빈도 분포양상을 나타내었다. 캘러스 형성률과 식물체 재분화 능력에 대해 추정된 광의의 유전력은 각각 72.8%와 82.7%로 추정되었다.
