Effect of Phytolacca americana Extracts on the Activities of AsPOX and GuPOX during Germination Process of Cassia mimosoides var. nomame

  • Lee, Ho-Joon (Department of Biological Sciences, Division of Natural Science, Konkuk University) ;
  • Kim, Yong-Ok (Department of Biological Sciences, Division of Natural Science, Konkuk University)
  • Published : 2000.04.01

Abstract

After Cassia mimosoides var. nomame was treated with Phytolacca americana extracts during the germination process, its effect on the activities of AsPOX and GuPOX were examined. As the concentration of P. americana extract increased. the activity of AsPOX decreased while that of GuPOX increased. During the germination process, the activity of AsPOX was lower than the control, while the activity of GuPOX was over 2 times higher than the control. Activity of total peroxidase by IEF was extremely high at pI 6.4 and pI 6.6 when treatment was made with a 30% concentration of P. americana extract. The treatment with phenolic compounds, caffeic acid and benzoic acid did not show much difference from the control although a slight increase was observed at pI 6.6. Activity of GuPOX in C. mimosoides was over 4 times higher in roots than in shoots. Namely, because GuPOX activity of C. mimosoides was increased by extracts of P. americana, defense enzyme, GuPOX. was generated against external stress, and we could certified the activity increase at pI 6.4, especially in root.

Keywords

References

  1. Free Radical Biol. and Med. v.23 Use of transgenic plants to study antioxidation defenses Allen,R.D.;R.P.Webb;S.A.Schake
  2. Antioxidants in higher plants Alscher,R.G;J.L.Hess
  3. Plant Cell Physiol. v.35 Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants Amako,K.;G.X.Chen;K.Asada
  4. Physiol. Plant. v.85 Ascorbate peroxidase: A hydrogen peroxide scavenging enzyme in plants Asada,K.
  5. Agron. J. v.76 Allelopathic effects of annual weed residues growth and nutrient uptake of corn and soybeans Bhowmik,P.C.;J.D.Doll
  6. Science v.209 Juvocimenes: potent juvenile hormone mimics from sweet basil Bowers.W.S.;R.Nishida
  7. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,M.M.
  8. Amer. Mid. Nat. v.90 Allelopathic effects of Rumex crispus on Amaranthus retroflexus, grain sorghum and field corn Einhellig,F.A.;J.A.Rasmussen
  9. Plant Physiol. v.81 Immunocytochemical localization and time course of appearance of an anionic peroxidase associated with suberization in wound-healing potato tuber tissue Espelic,K.E.;V.R.Franceschi;P.E.Kolattukudy
  10. Can. J. Plants Sci. v.57 Influence of seed size, protein content and cultivar on early seedling vigor in wheat Evans,L.E.;G.M,Bhatt
  11. Physiol. Plant. v.64 A two-step control of basic and acidic peroxidase and its significance on growth and develoment Gaspar,T.;C.Penel;F.J.Castillo;H.Greppin
  12. Plant Physiol. v.51 Hormonal control of isoperoxidase in lentil embryonic axis Gaspak,T.;A.A.Khan;D.Fires
  13. J. Chem. Ecol v.16 Allelopathy of small everlasting (Antennaria microphylla): Phytotoxicity to leaf spurge (Euphorbia esula) in tissue culture Hogan,M.E.;G.D.Manners
  14. Proc. Fourth North Amer. For. Biol. Workshop Allelopathic interference among plants. Ⅱ. Physiological models of action Horsley,S.B.
  15. Plant Physiol v.48 Evidence for osmotic regulation of hydroytic enzyme production in germinating barley seeds Jones,R.L.;J.E.Armstrong
  16. Plant Cell v.9 Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress Karpinski,S.;C.Escobar;B.Karpinski;G.Creissen;P.M.Mullineaux
  17. Plant Physiol. v.51 Embryoless wheat grain: A natural system for the study of gibberellin induced enzyme formation Khan,A.A.;R.Verbeek;E.C.Waters,Jr.;H.A. van Onckleen
  18. Phytochemisty v.19 Isolation and characterization of two isoperoxidases from tobacco tissue culture Kim,S.S.;S.H.Wender;E.C.Smith
  19. Physiol. Plant. v.97 Alterations in the antioxidative system of suspension-cultured soybean cells (Glycine max) induced by oxidative stress Knorzer,O.C.;J.Durner;P.Boger
  20. Plant Physiol v.84 Tissue specificity of tobacco peroxidase isoenzymes and their induction by wounding and tobacco mosaic virus infection Lagrimini,L.M.;S.Rothstein
  21. Plant Physiol v.96 Wound-induced deposition of polyphenols in transgenic plants overexpressing peroxidase Lagrimini,L.M.
  22. Korean J. Ecol. v.20 Allelopathic effects on seed germinaiton and fungus growth form the secreting substances of some plants Lee,H.J.;Y.O.Kim;N.K.Chang
  23. Effects of pine-produced chemicals on selected understory species in a Pinus ponderosa community Lodhi,M.A.K.;K.T.Killingbeck
  24. Amer. J. Bot. v.63 Role of allelopathy as expressed by dominating trees in a lowland forest in controlling the productivity and pattern of herbaceous growth Lodhi,M.A.K.
  25. Phytopathology v.55 Peroxidase activity in virus-infected sweet potatoes Loebenstein,G.;N.Linsey
  26. Plant Physiol v.97 Purification and characterization of pea cytosolic ascorbate peroxidase Mittler,R.;B.A.Zilinskas
  27. Plant Physiol v.115 Influence of salicylic acid on H₂O₂-metabolizing enzymes Rao,M.V.;G.Paliyath;D.P.Ormrod;D.P.Murr;C.B.Watkins
  28. Allelopathy (2nd ed.) Rice,E.L.
  29. J. Chem. Ecol v.7 Effects of decomposing rice straw on growth of and nitrogen fixation by Rhizobium Rice,E.L.;C.Y.Lin;C.Y.Huong
  30. J. Heredity v.5 Tissue specific isozyme variation in maize Scandalios,J.G.
  31. Adv. Genet. v.28 Response of plant antioxidant defense genes to environmental stress Scandalios,J.G.
  32. Plant and Cell Physiol v.222 Isozymes of glucose-6-phosphate dehydrogenase and NAD malate dehydrogenase in shoot forming foliar discs of tobacco Seeni,S.;A.Gnanam
  33. Plant Physiol v.45 Deuterium oxide as a density label of perioxidase in germination barley embryos Wayne,A.;J.V.Jacobsen;J.G.Scandalios;J.E.Varner
  34. Science v.171 Allelochemics: Chemical interactions between species Whittaker,R.H.;P.P.Feeny
  35. Korea Biochem. J. v.21 Purification and characterization of an anionic isoperoxidase from Korean radish root Yoo,W.I.;S.S.Kim