미생물학회지 (Korean Journal of Microbiology)

  • 제36권1호
  • /
  • Pages.58-63
  • /
  • 2000
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Klebsiella oxytoca C302의 분리 동정 및 방향족 탄화수소물질의 분해특성

Isolation and Identification of Klebsiella oxytoca C302 and Its Degradation of Aromatic Hydrocarbons

  • 김기필 (충북대학교 미생물학과) ;
  • 이정순 (충북대학교 미생물학과) ;
  • 박송이 (충북대학교 미생물학과) ;
  • 이문수 (생명공학연구소 유전자센터) ;
  • 배경숙 (생명공학연구소 유전자센터) ;
  • 김치경 (충북대학교 미생물학과)
  • 발행 : 2000.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

공업단지 폐수로부터 benzoate에 대하여 분해능을 보이는 균주를 분리하여 생화학적 특성과 세포 지방산 분석을 실시하여 동정한 결과 Klebsiella oxytoca로 밝혀졌다. 따라서 이 균주를 Klebsiella oxytoca strain C3O2라 명명하였으며 여러 가지 방향족 탄화수소에 대한 분해특성을 알아본 결과, benzoate 외에 catechol, protocatechuate, 4-hydroxybenzoate에 대한 분해능이 우수하였으며, 특히 benzoate와 catechol에 대하여 높은 분해능을 보였다. Klebsiella oxytoca C3O2 균주의 catechol 분해능에 대한 환경요소의 영향을 실험한 결과, $30^{\circ}C$와 pH 7.0에서 그리고 0.5~1.0 mM의 농도에서 왕성한 생장과 분해능을 보였다.

A bacterial isolate capable of degrading benzoate was selected from wastewater of Yocheon industrial complex and examined its biochemical characteristics and fatty acid composition. The isolate was identified as Klebsiella oxytoca strain C302. The strain C3O2 degraded catechol, protocatechuate, and 4-hydroxybenzoate as well as benzoate. The strain grew on and degraded 0.5 to 1.0 mM catechol most actively in MM2 medium at pH 7.0 and $30^{\circ}C$.

키워드

참고문헌

  1. Appl. Environ. Microbiol. v.55 Bacterial dehalogenation of chlorobenzoates and coculture biodegradation of 4,4'-dichlorobiphenyl Adriaens, P.;H.-P. E. Kohler;D. Kohler-Staub;D.D. Focht
  2. Appl. Environ. Microbiol. v.61 A meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorbiphenyl by Pseudomonas cepacia P166 Arensdorf, J. J.;D. D. Focht
  3. Analy. Chem. v.29 Determination of trace amounts of chlorine in naphtha Bergmann, J. G.;J. Sanik
  4. Microbiology v.140 The lower pathway operon for benzoate catabolism in biphenyl-utilizing Pseudomonas sp. strain IC and the nucleotide sequence of the bphE gene catechol 2,3-dioxgenase Carrington, B.;A. Lowe;L. E. Shaw;P.A. Williams
  5. J. Microbiol. v.35 no.4 Dechlorination of 4-chlorobenzoate by Pseudomonas sp. DJ-12 Chae, J. C.;C. K. Kim
  6. Microbiol. Rev. v.55 Biodegradation of halogenated organic compounds Chaudhry, G.R.;S. Chapalamadugu
  7. Microbiol. Rev. v.55 Microbiological degradation of pesticides in yard waste composting Fogarty, A.M.;O.H. Tuovinen
  8. FEMS Microbiol. Rev. v.103 Microbial breakdown of halogenated aromatic pesticides and related compounds $H\"{a}gblom$, M, M.
  9. J. Bacetriol v.173 Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xy/XYZ, genes encoding benzoate dioxygenases Harayama, S.;M. Rekik;A. Bairoch;E.L. Neidle;L. N. Ornston
  10. Appl. Environ. Microbiol. v.62 Phenotypic and genotypic characterization of phenanthrene-degrading fluorescent Pseudomonas biovars Johnsen K.;S. Andersen;C.S. Jacobsen
  11. J. Bacteriol v.176 Nucleotide sequence and functional analysis of the meta-cleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102 Kikuchi, Y.;Y. Yasukochi;Y. Nagata;M. Fukuda
  12. J. Microbiol. v.35 no.4 Characteristics of catechol 2,3-dioxygenase produced by 4-chlorobenzoate degrading Pseudomonas sp. S-47 Kim, K. P.;D. I. Seo;K. H. Min;J. O. Ka;Y. K. Park;C. K. Kim
  13. Biochem. Biophy. Res. Comm v.176 Dehalogenation of 4-chlorobenzoate dehalgenase from Pseudomonas sp. CBS3; an ATP/coenzyme A dependent reaction $L\"{o}ffler$, F.;R. $M\"{u}ller$;F. Lingens
  14. J. Bacteriol v.169 Cloning and expression in Escherichia coli of Acinetobacter calcoaceticus genes for benzoate degradation Neidle, E.L.;M.K. Shapiro;N. Ornston
  15. J. Bacteriol v.164 Morphological forms and viability of Campylobacter species studied by electron microscopy Ng, L.K.;R. Sherburne;D.E. Taylor;M. E. Stiles
  16. J. Bacteriol v.142 Hybrid pathway for chlorobenzoate metabolism Pseudomonas sp. B13 derivatives Reineke, W.;H. J. Knackmuss
  17. Ann. Rev. Microbiol v.42 Microbial degradation of haloaromatics Reineke. W.
  18. J. Microbiol. v.35 no.3 Isolation of Pseudomonas sp. S-47 and its degradation of 4-chlorobenzoic acid Seo, D. I.;J. Y. Lim;Y. C. Kim;K. H. Min;C. K. Kim
  19. FEMS Microbiol. Lett. v.41 Initial characterization of 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 Thiele J.;R. $M\"{u}ller$;F. Lingens
  20. J. Bacteriol v.171 Cloning, expression, and regulation of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase genes Zylstra, G. J.;R. H. Olsen;D. P. Ballou