Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Identification of Klebsiella oxytoca C302 and Its Degradation of Aromatic Hydrocarbons

Klebsiella oxytoca C302의 분리 동정 및 방향족 탄화수소물질의 분해특성

  • 김기필 (충북대학교 미생물학과) ;
  • 이정순 (충북대학교 미생물학과) ;
  • 박송이 (충북대학교 미생물학과) ;
  • 이문수 (생명공학연구소 유전자센터) ;
  • 배경숙 (생명공학연구소 유전자센터) ;
  • 김치경 (충북대학교 미생물학과)
  • Published : 2000.03.01
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Abstract

A bacterial isolate capable of degrading benzoate was selected from wastewater of Yocheon industrial complex and examined its biochemical characteristics and fatty acid composition. The isolate was identified as Klebsiella oxytoca strain C302. The strain C3O2 degraded catechol, protocatechuate, and 4-hydroxybenzoate as well as benzoate. The strain grew on and degraded 0.5 to 1.0 mM catechol most actively in MM2 medium at pH 7.0 and $30^{\circ}C$.

공업단지 폐수로부터 benzoate에 대하여 분해능을 보이는 균주를 분리하여 생화학적 특성과 세포 지방산 분석을 실시하여 동정한 결과 Klebsiella oxytoca로 밝혀졌다. 따라서 이 균주를 Klebsiella oxytoca strain C3O2라 명명하였으며 여러 가지 방향족 탄화수소에 대한 분해특성을 알아본 결과, benzoate 외에 catechol, protocatechuate, 4-hydroxybenzoate에 대한 분해능이 우수하였으며, 특히 benzoate와 catechol에 대하여 높은 분해능을 보였다. Klebsiella oxytoca C3O2 균주의 catechol 분해능에 대한 환경요소의 영향을 실험한 결과, $30^{\circ}C$와 pH 7.0에서 그리고 0.5~1.0 mM의 농도에서 왕성한 생장과 분해능을 보였다.

Keywords

References

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