Molecular Systematics of Rhizoctonia solani Isolates from Various Crops with AFLP

AFLP를 이용한 Rhizoctonia solani 균주들의 분자계통학적 특성규명

  • 최혜선 (인천대학교 자연과학대학 생물학과) ;
  • 김경수 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 김희종 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 이윤수 (강원대학교 농업생명과학대학 식물응용과학부)
  • Published : 2000.03.01

Abstract

Rhizoctonia solani Kuhn[Thanatephorus cucumeris (Frank) Donk]is a destrutive soil-borne plant pathogen affecting many agricultural crops worldwide. R. solani is divided into anastomosis groups based on the ability of the hyphae to fuse, and into subgroups based on morphological, physiological characteristics. AG classifications are convenient and useful in identifying primary causal agents of Rhizoctonia diseases, although the mechanism of anastomosis is not fully understood. Beacause of the difficulties, we sought to develop a more direct method for genetic identification and charaterization of R. solani. Twenty nine isolates of R. solani were used for the analysis of genetic relationships among themselves and for rapid anastomosis grouping with AFLP method. All isolates studied were divided into five groups. Isolate 6 was included in AG-3 with 67% genetic similarity. When isolates 3 was compared with 13 and 10 each, they showed more than 84% and 83% similarity, respectively. Isolates 3, 4, 5, 13, and 16 were included in AG-1 with 83% genetic similarity. Isolates 1, 7, and 8 were included in AG-1(IB).

Rhizoctonia solani 분류를 위해 균사의 anastomosis group과 phylogenetic group간의 유전적 차이의 분석을 위해 AFLP를 실시하였다. 그 결과, 각 집단의 유사도는 크게 5집단으로 나뉘어졌다. 균주 9와 균주 20은 0.777을 나타내며 AG-5 group에 속하였고 균주 6은 균주 24와 0.670으로 AG-3 group에 속하였다. 균주 3은 균주 13과 0.844, 균주 10과 0.833의 높은 상동성을 보이며 AG-2-2 group에 속하였다. 균주 2는 균주 11과 0.744, 균주 3은 균주 13과 0.844, 균주 4는 균주 5와 0.870, 균주 5는 균주 16과 0.833을 나타내며 AG-1 group에 속하였다. 균주 7은 균주 8과 0.837의 높은 상동성을 나타내고, 균주 8은 AG-1( I B)와 0.751를 나타냈고, 균주 1과 균주 7,8은 AG-1( I B)에 속하였다.

Keywords

References

  1. Phytophathology v.69 Serological relationships among anastomosis group of Rhizoctonia solani Adams, G.S., Jr.;E.E. Butler
  2. Rhizoctonia solani: Biology and Pathology The genetics and pathology of Rhizoctonia diseases and their occurrence Anderson, N.A.;Parmeter J.R.Fr.(ed.)
  3. Annu. Rev. Phytopathol v.20 The genetics and pathology of Rhizoctonia solani Anderson, N.A.
  4. Mol. Physiology v.1 Evolutionary relationships within fungi; Analysis of nuclear small subunit rRNA sequences Bruns, T.D.;R. Vilgalys;S.M. Barns;D. Gonzalez;D.S. Hibbett;D.J.Lane;L.Simon;S.Stickel;T.M. Szaro;W.G. Weisburg;M.L. Sogin
  5. Mycologia v.72 Anastomosis groups among isolates of Ceratobasidium cornigerum and related fungi Burpee, L.L.;P.L. Sanders;H. Cole,Jr
  6. Phytopathology v.77 Characterization of a new anastomosis group (AG 9) of Rhizoctonia solani. Carling, D.E.;R.H. Leiner;K. M. Kebler
  7. Korean J. Microbiology v.35 Molecular systematics of Rhizoctonia solani isolates from various crop with RFLP and PCR-RFLP Choi, H.S.;S.J. Woo;W.S. Shin;H.J. Kim;K.S. Kim;Y.S. Lee
  8. Phytopathology v.81 Characterization of anastomosis groups of binucleate Rhizoctonia spp. using restriction analysis of an amplified ribosomal RNA gene Cubeta, M.A.;E. Echandi;T. Abernethy;R. Vilaglys
  9. Korean J. Mycology v.26 Analyses of genic relationships of R. solani isolates from various crop species and rapid identification of AGs of R. solani with RAPD method Lee, Y.S.;H.S. Choi;S.J. Woo;K.S. Kim;M. J. Kim;J.O. Shim;M.W. Lee
  10. Can. J. Plant Pathol v.12 Genetic relationship among isolates of Rhizoctonia solani anastomosis group-2 based on isozyme analysis Liu, Z.L.;D.L. Nicksrent;J.B. Sinclair
  11. Mol. Ecol. v.5 Amplified fragment length polymorphism(AFLP) fingerprinting of symbiotic fungi cultured by the fungus-growing ant Cyphomyrmex minutus Muller, U.G.;S.E. Lipari;M.G. Milgroom
  12. Can. J. Plant Pathology v.12 Investigation of genetic relatedness among anastomosis groups of Rhizoctonia solani using cloned DNA probes Jabaji-Hare,S.H.;Y. Meller;S. Gill;P.M. Charest
  13. Ann Phytopathol. Soc. Jpn. v.45 Some properities of anastomosis group 6 and BI in Rhizoctonia solani Kuhn Kuninaga, S.;R. Yokosawa;A. Ogoshi
  14. Ann Phytopathol. Soc. Jpn. v.46 A comparison of DNA composition among anastomosis groups in Rhizoctonia solani Kuhn Kuninaga, S.;R. Yokosawa
  15. Ann Phytopathol. Soc. Jpn. v.48 DNA base sequence homology in Rhizoctonia solani Kuhn.Ⅰ . Genetic relatedness within anastomosis group 1. Kuninaga, S.;R. Yokosawa
  16. Ann Phytopathol. Soc. Jpn. v.48 DNA base sequence homology in Rhizoctonia solani Kuhn.Ⅱ. Genetic relatedness within anastomosis group 2. Kuninaga, S.;R. Yokosawa
  17. Ann Phytopathol. Soc. Jpn. v.50 DNA base sequence homology in Rhizoctonia solani Kuhn.Ⅳ. Genetic relatedness within AG 4 Kuninaga, S.;R. Yokosawa
  18. Trans. Br. Mycol. Soc. v.85 Anastomosis grouping of some isolates of Thanatephorus cucumeris from agricultural soils in south Australia Neate, S.M.;J.H. Warcup
  19. Annu. Rev. Phytopathol v.25 Ecology and pathologenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kuhn Ogoshi, A.
  20. Trans. Mycol. Soc. Jpn v.26 The perfect state of Rhizoctonia oryzae and R. zeae and the anastomosis groups of Waitea circinata Oniki, M.;A. Ogoshi;T. Araki;R. Sakai;S. Takana
  21. Rhizoctonia solani: Biology and Pathology Taxonomy and nomenclature of the imperfect state Parmeter, J.R. Jr;H.S. Whitney;J. R. Parmeter(ed.)
  22. Phytopathology v.59 Anastomosis grouping among isolates of Thanatephorus cucumeris Parmeter, J.R.;Jr. R.T. Sherwood;W.D. Platt
  23. Phytopathology v.73 Comparison of anastomosis group of Rhizoctonia solani by polyacrylamide gel electrophoresis of soluble proteins Reynold, M.;A.R. Weinhold;T.J. Morris
  24. NTSYS-pc: Numerical taxonomy and multivariate analysis system. (version 1.70) Rohlf, F.J.
  25. Phytopathology v.59 Morphology and physiology in four anastomosis groups of Thanatephorus cucumeris Sherwood, R.T.
  26. Can. J. Bot. v.53 Morphology, develop-ment, and cytochemistry of the hypae and sclerotino of species in the Rhizoctonia complex Tu, C.C.;J.W. Kimbrough
  27. Bot. Gaz. v.139 Systematic and phylogeny of fungi in the Rhizoctonia complex. Tu, C.C.;J.W. Kimbrough