YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Curcumin on the Microglial Activation

Curcumin이 microglia의 활성화에 미치는 영향

  • 정기경 (식품의약품안전청 국립독성연구소 약리부) ;
  • 이상진 (성균관대학교 생명자연자원과학대학) ;
  • 이선우 (식품의약품안전청 국립독성연구소 약리부) ;
  • 강석연 (식품의약품안전청 국립독성연구소 약리부) ;
  • 김태균 (식품의약품안전청 국립독성연구소 약리부) ;
  • 강주혜 (식품의약품안전청 국립독성연구소 약리부) ;
  • 홍성렬 (성균관대학교 생명자연자원과학대학) ;
  • 주일로 (아주대학교 의과대학) ;
  • 김승희 (식품의약품안전청 국립독성연구소 약리부)
  • Published : 2000.10.01
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Abstract

Microglia, brain resident macrophages, play a central role in the inflammatory responses of the brain and are activated in brain injuries and several neurodegenerative diseases such as Alzheimer's and Parkinson's disease, thereby aggravating the course of these diseases. In this study, the effects of plantderived compounds such as curcumin or gingerol on the microglial activation were examined. Microglial cultures were prepared from 2~3 week mixed primary glial cultures obtained from the cerebral cortex of 1~2 day old rats and identified by immunocytochemistry using microglial-specific antibody OX-42. Microglia were activated by lipopolysaccharide (LPS) and interferon-${\gamma}$ (IFN-${\gamma}$) and the effect of curcumin or 6-gingerol on the microglial activation was examined. Specific parameters measured to monitor microglial activation were nitric oxide (NO), prostaglandin E$_2$(PGE$_2$) and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) release. Curcumin (1~10 $\mu$M) inhibited NO release induced by LPS and IFN-${\gamma}$ in a dose-dependent manner whereas 6-gingerol (2~20 $\mu$M) did not have any effect on LPS/IFN-${\gamma}$-induced NO release. The levels of PGE$_2$and TNF-$\alpha$ induced by LPS and IFN-${\gamma}$ were also inhibited by 1~10 $\mu$M curcumin in a dose-dependent manner. These results showed that curcumin could modulate microglial activation.

Keywords

References

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