YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Astragali Radix and Polygalae Radix on Cerebral Ischemic and Reperfused Injury

황기와 원지분획의 뇌허혈에 관한 약효연구

  • Han, Suk-Hee (College of Pharmacy, Sungkyunkwan University) ;
  • Park, Jin-Hyuk (College of Pharmacy, Sungkyunkwan University) ;
  • Kim, Jin-Sook (Korea Institute of Oriental Medicine) ;
  • Lee, Sun-Mee (College of Pharmacy, Sungkyunkwan University)
  • 한석희 (성균관대학교 약학대학) ;
  • 박진혁 (성균관대학교 약학대학) ;
  • 김진숙 (한국한의학연구원 연구부) ;
  • 이선미 (성균관대학교 약학대학)
  • Published : 2000.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to investigate the pharmacological properties of fractions of Astragali Radix and Polygalae Radix, the effects of the fractions on cerebral ischemia and subsequent reperfusion were studied. Brain ischemia was induced by bilateral common carotid artery occlusion in mongolian gerbil. Brains were recirculated for 30 mins after the 20 min occlusion. Methanol and butanol fractions of Astragali Radix and Polygalae Radix were administered orally 2 hrs before common carotid artery occlusion. Histological observations showed that brain ischemia induced severe brain damage evidenced by the presence of necrotic foci, edema and hemorrhage. This injury was prevented by the methanol fraction and butanol fraction of Polygalae Radix. The level of ATP in brain tissue significantly decreased in ischemic gerbils. This decrease was prevented by the pretreatment with butanol fraction of Polygalae Radix. In contrast, the levels of lactate and lipid peroxide were both elevated in ischemic gerbils. This elevation was inhibited by the pretreatments with methanol fraction and butanol fraction of Polygalae Radix. Our findings suggest that the Polygalae Radix improves ischemia-induced brain damage.

Keywords

References

  1. 도해향약(생약) 대사전(식물편) 정보섭;신민교
  2. 中藥大辭典 相賀撤夫
  3. 약학회지 v.23 황기의 혈압강하 작용에 관한 연구 임동윤
  4. 약학회지 v.40 황기의 메탄올 추출물의 용량에 따른 면역생물학적 연구 김정훈;박정숙;채병숙;강태욱;박찬봉;안영근
  5. 아세아 생약도감 육창수
  6. The Pharmacology of Chinese Herbs Huang KeeChang
  7. 동의보감 허준
  8. Stroke v.17 Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantifacation of experimental cerebral infarction in rats Bederson, J.B.;Pitts, L.H.;Germano, S.M.;Nishimura, M.C.;Davis, R.L.;Bartkowski, H.M.
  9. Am. J. Med. v.91 no.sup. 3C Myocardial reperfusion injury;Mechanism and Management Giuseppe, A.;Massimo, C.
  10. Methods of Enzymatic Analysis Determination with lactate dehydrogenase and NAD Gutmann, I.;Wahefeld, A.W.
  11. Intervat. J. Vit. Nutri. Res. v.46 Effect of vitamin E deficiency on the level of superoxide dismutase, glutathione peroxidase, catalase and lipid peroxide in rat liver Masugi, F.;Nakamura, T.
  12. Anal. Biochem. v.72 A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the pirinciple of protein dye binding Bradford, M.M.
  13. Neuroscience v.9 Amino acid transmitters and their pathways in the mammalian central nervous system Fagg, G.E.;Foster, A.C.
  14. Neuropathol. Appl. Neurobiol. v.7 Regional neuron damage after cerebral ischemia in normo- and hypoglycemic rats Diemer, N.H.;Siemkowicz, E.
  15. Acta Neuropathol.(Berl) v.32 Experimental ischemia in mongolian gerbils Ⅰ. light microscopic observation Ito, U.;Spatz, M.;Walker, J.T. Jr.;Klatzo, I.
  16. Brain Res. v.239 Delayed neuronal death in gerbil hippocampus following ischemia Kirino, T.
  17. Neurology v.32 Moderate hypoglycemia augments ischemic brain damage;a neuropathologic study in the rat Pulshinelli, W.A.;Brierly, J.B.;Plum, F.
  18. Anesthesiol. v.79 The effect of a platelet activating factor antagonist(BN52021) on neurologic outcome and histopathology in a canine model of complete cerebral ischemia Hofer, R.E.;Christopherson, T.J.;Scheithauer, B.W.;Milde, T.H.;Lanier, W.L.
  19. Stroke v.23 Neuroprotective effects of carvediol, a new antihypertensive agent, in cultured rat cerebellar neurons and in gerbil global brain ischemia Lysko, P.G.;Lysko, K.A.;Yue, T.;Webb, C.L.;Gu, J.;Feurstein, G.
  20. Ital. J. Neurol. Sci. v.2 no.sup. A new model of focal brain ischemia based on the intracerebral injection of endothelin-1 Agnati, L.F.;Zoli, M.;Kurosawa, M.;Benfenati, F.;Biagini, G.;Zini, I.;Hallstrom, A.;Ungerstedt, U.;Toffano, G.;Fuxe, K.
  21. J. Cereb. Blood Flow Metab. v.1 Facal cerebral ischemia in the rat;description of technique early neuropathological consequences following middle cerebral artery occlusion Tamura, A.;Graham, D.I.;McCulloch, J.;Teasdll, G.M.
  22. J. Cereb. Blood Flow Metab. v.10 Cerebral blood flow, glucose utilization, regional glucose, and ATP content during the maturation period of delayed ischemic injury in gerbil brain Mies, G.;Paschen, W.;Hossmann, K.A.
  23. J. Neurochem. v.28 The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization;theory, procedure, and normal values in the conscious and anesthetized albino rat Sokoloff, L.;Reivich, M.;Kennedt, C.;Des Rosiers, M.H.;Shinohara, M.
  24. Stroke v.18 Effect of 5-minute ischemia on regional pH and energy state of the gerbil brain;relationship to selective vulnerability of the hippocampus Munekata, K.;Hossmann, K.A.
  25. J. Cereb. Blood Flow Metab. v.1 Brain lactic acidosis and ischemic cell damage;2. Histopathology Kalimo, H.;Rehncrona, S.;Soderfeldt, B.;Olsson, Y.;Siesjo, B.K.
  26. Stroke v.21 Relationship between plasma glucose, brain lactate, and intracellular pH during cerebral ischemia in gerbils Comb, D.J.;Dempsey, R.J.;Maley, M.;Donaldson, D.;Smith, C.
  27. J. Cereb. Blood Flow Metab. v.6 Changes in extra- and intracelluar pH in the brain during and following ischemia in hyperglycimic and in moderately hypoglycemic rats Smith, M.L.;Von Hanwehr, R.;Siesjo, B.K.
  28. Stroke v.17 Glycolytic inhibition by 2-deoxyglucose reduces hyperglycemia-associated mortality and morbidity in the ischemic rat Comb, D.J.;Reuland, D.S.;Martin, D.B.;Zelenock, G.B.;D'Alecy, L.G.
  29. Stroke v.19 21-Aminosteroid lipid peroxidation inhibitor U7006F protects against cerebral ischemia in gerbils Hall, E.D.;Pazara, K.E.;Braughler, J.M.
  30. Anestheisology v.67 The effects of superoxide dismutase on neurological recovery (Cerebral blood flow and evoked potentials) following global cerebral ischemia(abstract) Snelling, L.K.;Ackerman, A.D.;Dean, J.M.;North, M.C.;Traystman, R.J.