YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Effects of Somatid on the Cytotoxicity of Cancer Cells and Human Papillomavirus Type 16 E6 and E7 Oncogenes

생기액(生肌液)의 세포독성 및 자궁경부암 바이러스 (HPV 16 type) 암 유발인자 E6와 E7의 작용에 미치는 효과

  • Joung, Ok (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Cho, Young-Sik (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Cho, Cheong-Weon (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Lee, Kyung-Ae (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Shim, Jung-Hyun (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Cho, Min-Chul (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Lee, Hong-Soo (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Yeom, Young-Il (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Kim, Sang-Bom (SB Research Institute) ;
  • Park, Sue-Nie (Department of Viral Products, KFDA) ;
  • Yoon, Do-Young (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology)
  • 정옥 (생명공학연구소) ;
  • 조영식 (생명공학연구소) ;
  • 조정원 (생명공학연구소) ;
  • 이경애 (생명공학연구소) ;
  • 심정현 (생명공학연구소) ;
  • 조민철 (생명공학연구소) ;
  • 이홍수 (생명공학연구소) ;
  • 염영일 (생명공학연구소) ;
  • 김상범 (SB 식품의학연구소) ;
  • 박순희 (식품의약품 안전청 생물학평가부 바이러스 제제과) ;
  • 윤도영 (생명공학연구소)
  • Published : 2000.08.01
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Abstract

Cervical cancer is one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year. A strong association between certain human papilloma viruses (HPV types 16 and 18) and cervical cancer has been well known. An extract of natural products, named as Somatid, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16. This Somatid inhibited the proliferation of human cervical cancer cell lines (C-33A, SiHa, CaSki) and HaCaT keratinocytes in a dose response manner, In vitro binding assay and ELISA showed that Somatid inhibited the in vitro biding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, Somatid inhibited the in vitro binding of E7 and Rb which is essential tumor suppressor for the control of cell cycle. The levels of mRNA for E6 and E7 were also decreased by Somatid. Our data suggested that Somatid inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus can be used as a putative anti-HPV agent for the treatment of cervical carcinomas caused by HPV.

Keywords

References

  1. 圖解本草綱目 李時珍(著)
  2. Proc. Natl. Acad. Sci. v.88 The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines Scheffner, M.;Munger, K.;Byrne, J.C.;Howley, P.M.
  3. Mol. Carcinog. v.14 Analysis of oncogenes, tumor suppressor genes, autocrine growth-factor production, and differentiation state of human osteosarcoma cell lines Isfort, R.J.;Cody, D.B.;Lovell, G.;Doersen, C.J.
  4. J. Cell. Biochem. v.66 Expression of cellular genes in HPV16-immortalized and cigarette smoke condensate-transformed human endovervical cells Yang, X.;Nakao, Y.;Pater, M.M.;Tang, S.C.;Pater, A.
  5. J. Virol. v.74 LMP1 of epstein-barr virus induces proliferation of primary mouse embryonic fibroblasts and cooperatively transforms the cells with a p16-insensitive CDK4 oncogene Yang, X.;Sham, J.S.;Ng, M.H.;Taso, S.W.;Zhang, D.;S.W.L; Cao, L.
  6. Oncogene v.18 Viral oncogenes accelerate conversion to immortality of cultured conditionally immortal human mammary epithelial cells Garbe, J.;Wong, M.;Wigington, D.;Yaswen, P.;Stampfer, M.R.
  7. J. Viral Hepat. v.3 Hepatitis B virus transcriptional activators;mechanisms and possible role in oncogenesis Henkler, F.F.;Koshy, R.
  8. Cytometry v.35 Cell cycle models for molecular biology and molecular oncology;exploring new dimensions Shackney, S.E.;Shankey, T.V.
  9. Int J. Oncol. v.15 The level of tyrosine kinase activity regulates the expression of p21/WAF1 in cancer cells Reddy, K.B.;Deshamouni, V.G.;Chen, Y.Q.
  10. Bioessays v.17 Human papillomavirus E6 and E7;proteins which deregulate the cell cycle Tomasino, M.;Crawford, L.
  11. Oncogene v.7 Recombinant retroviruses encoding human papillomavirus type 18 E6 and E7 genes stimulate proliferation and delay differentiation of human keratinocytes early after infection Woodworth, C.D.;Cheng, S.;Simpson, S.;Hamacher, L.;Chow, L.T.;Broker, T.R.;Dipaolo, J.A.
  12. Science v.243 The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product Dyson, N.;Howley, P.M.;Munger, K.;Harlow, E.
  13. Nucleic Acids Res. v.19 no.5 Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products Marchuk, D.;Drumm, M.;Saulino, A.;Collins, F.S.
  14. Antiviral Res. Development of screening systems for drugs against human papillomvirus-associated cervical cancer;based on E6-E6-AP binding Cho, Y.S.;Cho, C.W.;Joung, O.;Lee, K.A.;Park, S.N.;Yoon, D.Y.
  15. Carcinogenesis v.17 The human papillomavirus E6/E7 genes induce discordant changes in the expression of cell growth regulatory proteins Pei, X.F.
  16. J. Natl. Cancer Institute v.91 no.14 Potential Drugs Against Cervical Cancer;Zinc-Ejecting Inhibitors of the Human Papillomavirus Type 16 E6 Oncoprotein Beerheide, W.;Bernard, H.U.;Tan, Y.J.;Ganesan, A.;Rice, W.G.;Ting, A.E.