YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Cytotoxicity of SD-994 from Artemisia argyi against L1210 Cells with Concomitant Induction of Antioxidant Enzymes

황해쑥 추출정제물 SD-994의 L1210암세포에 대한 세포독성과 항산화효소의 유발

  • 정대영 (상명대학교 자연과학부 화학전공) ;
  • 하혜영 (상명대학교 자연과학부 화학전공) ;
  • 김안나 (상명대학교 자연과학부 화학전공) ;
  • 이승민 (상명대학교 자연과학부 화학전공) ;
  • 민태진 (동국대학교 이과대학 화학과) ;
  • 박시원 (상명대학교 자연과학부 화학전공)
  • Published : 2000.06.01
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Abstract

SD-994 was prepared from methanol extract of Artemisia argyi by stepwise purification of solvent partioning and silica gel chromatography. In the course of this purification, fractions obtained at each step were investigated for their cytotoxicities against L1210 cells. Fractions A~G prepared from chloroform fraction showed considerable cytotoxicities raging 40~90% against L1210 cells. Subfractions I~IX obtained from fraction A exhibited various cytotoxicities and subfraction I (SD-994) was found to be the most effective compound. $IC_{50}$ values of SD-994 were measured to be $0.5{\;}{\mu\textrm{g}}/ml and less than $0.05{\;}{\mu\textrm{g}}/ml against L1210 cells and normal lymphocytes, respectively: When SD-994 was added to L1210 cell as cytotoxic agent, significantly increased amount of superoxide ($O_2^-$) and dramatically augmented activities of superoxide dismutase (SOD), specially MnSOD and glutathione peroxidase (GPx) were observed according to the concentration and incubation time. Whereas, in case of normal lymphocytes under the same condition, cytotoxicities were not apparent and the generation of superoxide ($O_2^-$) or the activity changes of SOD and GPx were insignificant. These results together indicate that the cytotoxic action of SD-994 against L1210 cell may be achieved via necrosis and/or apoptosis induced by reaction oxygen species which could not probably be completely abolished even by drastically increased antioxidant enzymes, SOD and GPx activities.

Keywords

References

  1. Yakhak Hoejj v.43 Cytotoxicity of Artemisia argyi extract against H9 cell and antioxidant enzymes Kim. K.H.;Jung. D.Y.;Min. T.J.;Park. S.W.
  2. J. Biochem. Molecular Biol. v.32 Antiproliferative effect of artemisia argyi extract aginst J774A. 1. cells and subcellular superoxide dismutase(SOD) activity changes Lee. T.E.;Park. S.W.;Min. T.J.
  3. Shoyakugaku Zasshi v.38 Studies on dental caries prevention by traditional chinese medicines (Part IV)Screening of crude drugs for anti-plaque action and effects of artemisia capillaries spikes on adherence of Streptococcus mutants to smooth surfaces and synthesis of glucan by glucosytransferease Namba. T.;Tsunezuka. M.;Takehana. Y.;Nunome. S.;Takeda. K.;Shu. Y.Z.;Kakiuchi. N.;Takgi. S.;Hattori. M.
  4. J. Natural Products v.56 A rapid screening method for artemisin and its congencers using MS/MS: searching for new analogues in Artemisia annua Ranashinghe. A.;Sweatlock. J.D.;Coos. R.G.
  5. Shoyakugaku Zasshi v.43 Efectf of mineral fertilizers on the productioj of flower bud and ch9oleretic substances in flower bud of artemisia capilaries Ikenaga. T.;Tajima. M.;Matuo. H.
  6. J. appl. Phamacol. v.5 Studies on protective effect of DA-9601, an Artemisia extract, against ethanol induced gastrci mucosal damage and its mechanism Oh. T.Y.;Ahn. B.Y.;Ko. J.I.;Ryu. b.K.;Son. M.W.;Kim. S.H.;Kim. W.G.;Lee. E.B
  7. J. Pharmaceut. Sci. v.68 Anti -bacterial activity of Artemisa herbaalba Yashphe. J.;Segal. R.;Breuerm. A.;Erdreich-Naftali. G.
  8. Int. J. Immunopharmac. v.11 Immunological mechanisms of antitumor activity of some kind of crude drugs on tumor necrosis factor production Xu. Q.;Mori. H.;Sakamoto. O.;Uesugi. Y.;Koda. A.
  9. Jap. J. Pharmacol. v.49 Mechnisms of antitumor activity of aqueous extract from chinese herbs; their immunopharmacological properties Mori. H.;Xu. Q.;Sakamoto. O.;Uesugi. Y.;Koda. A.;Nishioka. I.
  10. Free radical and oxidation phenomena in biloogical systems Definitions, properties and reactions of radicals Roberbroid. M.;Calderon. P.B.
  11. Prog. Lipid Res. v.32 Free radical lipid interactions and their pathological consequences Rice evance. C.;Burdon. R.
  12. Toxicol. Indus. Health v.9 Tissue injury by free radicals Chessman. K.H.
  13. The FASEB J v.9 Oxidative process and antioxidative defense mechanism in the aging brain Reitter. R.
  14. Bilogy and Medicine v.23 Oxidative stress hypothesis in Alzheimer disease Free radical Markesbery. W.R.
  15. Environ. Health Persp v.106 The role of oxidative stress in chemical carcinogenesis Klaunig. J.E.;Xu;Yong;Isemberg. J.;Bachowski. S.;Kolaja. K.L.;Jiang. J.;Stevenson. D.E.;Walborg. E.F.
  16. Free Radical Biologyand Medicine v.23 Oxidative stress hypothesis in Alzheimer disease Markesbery. W.R.
  17. Oxidative processes and antioxidants Urshini. F.;Selenium dependent peroxidase(ed) Paolletti. R.;Samu - ellson. B.;Cataparo. A.L.;poli. A.;Rinnetti. N.
  18. Int. J. Cancer v.51 SOD₂: new type of tumor suppressor gene? Bravard. A.;Sabatier. L.;Hofschir. F.;Ricoul. M.;Luccini. C.;Dutrilaux. B.
  19. Cancer Chemother. Rep. v.3 Cell culture screen, KB. Protocol 1600 National Cancer Institute USA
  20. Scan. J. Clin. Invest. v.21 Isoation of leukocytes from humman blood. Boyum. A.
  21. J. Biol. Chem. v.244 Superoxide dismutase assay McCord. J.M.;Fridovich. I.
  22. Biochem. Bipphys. Res. Commum v.77 Comparative study of superoxide dismutase, catalase and glutathione peroxidase levels in erythrocytes of different animals Maral. J.;Puget. K.;Michelson. A.M.
  23. In Free Radicals in Aging Age related alterations in antioxidative defense Matsuo. M.;Yu. B.P.(ed.)
  24. FEBS lett. v.358 Persistent oxidative stress in cancer Yoyokuni. S.;Okamoto. K.;Yodoi. J.;Hiai. H.
  25. Mayo Clin. Proc. v.63 Free radicals in medicine Southem. P.A.;Powis. G.
  26. Lancet v.344 Oxidative stress and cancer. Cerutti. P.A.
  27. FEBS Letters v.404 Role of peroxide and superoxide anion during tumor cell apoptosis Goman. A.;McGowan. A.;Gotter. T.G.
  28. Proc. Natl. Acad. Sci USA v.93 Reactive oxygen species are downstream mediators of p53 dependent apoptosis Johnson. T.M.;Yu.;Zu-Xi;Ferrans. V.J.;Lowens- tein. R.A.;Finkel. T.
  29. Cancer Res. v.59 Apoptosis, p53 and tumor cell sensitivity to anticancer agents Brown. J.M.;Wouters. B.G.
  30. Oncogene v.18 Mitochondrial DNA detemines the cellular response to cancer therapeutic agents Sinh. K.K;Russel. J.;Sigala. B.;Zhang. Y.;Williams. J.;Keshav. K.F.
  31. Br. J. Cancer. v.81 Cellular uptake, cytotoxicity and DNA binding studies of the novel imidazoacridinone antineoplastic agent C1311 Burger. A.;Jenkins. T.;Double. J.;bibby. M.;
  32. Cancer Res. v.57 Drug-induced apoptosis with enhanced fas (Apo-l/CD95) signaling in human T-acute lymphatic leukemia cells Villunger. A.;Egle. A.;Kos. M.;Hartman. B.;Geley. S.;Kofler. R.;Greil. R.