Relationship between Heat Shock Protein 70 Synthesis and Development of Thermotolerance in the Olive Flounder, Paralichthys olivaceus

  • Kim, Woo-Jin (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Myong, Jeong-In (Aquaculture Division, National Fisheries Research and Development Institute) ;
  • Lee, Jeong-Ho (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Park, Jung-Youn (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Park, Jung-Youn (Aquaculture Division, National Fisheries Research and Development Institut) ;
  • Kim, Doo-Won (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Kang, Kyung-KIl (Department of Molecular Biology, Pusan National University) ;
  • Kim, Han-Do (Department of Molecular Biology, Pusan National University)
  • Published : 2000.06.01

Abstract

Relationship between hsp 70 expression and development of thermotolerance in the olive flounder was investigated by exposing the fish to 23 or 26$^{\circ}C$ for 1 h. After selected intervals, they were re-exposed to 31$^{\circ}C$ for a duration of 15 min. Theremotolerance rapidly developed with increasing interval and peaked at 3 h interval. Subsequently, it gradually decayed and disappeared by 24 h interval. The flounder displayed remarkably high levels of hsp 70 mRNA and protein, as compared to control flounder. Hence, the olive flounder acquires thermotolerance, which is positively correlated with the synthesis of hsp 70.

Keywords

References

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