Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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A Study on the Formation of Vesicle by Chitosan Oligosaccharide Derivative

키토산 올리고당 유도체의 베지클 형성에 관한 연구

  • Published : 2000.06.01
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Abstract

Chitosan oligosaccharide with an average degree of polymerization 2-3 was prepared by degradation reaction using sodium nitrite, and the resulting aldehyde group was reduced to hydroxy group. N-palmitoyl, O-palmitoyl chitosan oligosaccha was obtained from the reaction between palmitoyl chloride and oligosaccharide under DMAP catalyst. Alkaline hydrolysis was carried out to remove the O-palmitoyl group. After dispersion and ultrasonication in aqueous solution, N-palmitoyl chitosan oligosaccharide gives vesicle structure. Its structure and size was analyzed by TEM.

게 껍질에서 얻어진 키토산에 아질산나트륨을 가한 후 수산화붕소산 나트륨으로 환원시켜 중합도 2∼3인 2,5-anhydro-D-mannitol의 말단기를 갖는 키토산 올리고당을 합성하였다 염화팔미틴산을 DUP 촉매하에서 키토산 올리고당과 반응시켜 키토산 올리고당에 세 개의 아실기가 결합된 키토산 올리고당 유도체를 얻었으며, 이를 다시 수산화칼륨으로 가수분해하여 N-팔미토일 키토산 올리고당을 합성하였다. 최종적으로 수용액에 분산시킨 후 초음파를 가한 후 형성된 베지클을 TEM 을 이용하여 확인할 수 있었다.

Keywords

References

  1. Advances in Chitin and Chitosan Brine, C.J.;Sandford, P.A.;Zikakis, J.P.
  2. Pure Appl. Chem. v.A31 Tokura, S.;Itoyama, K.;Nishi, N.;Nishimura, S.I.;Saiki, I.;Azuma, I.
  3. J. Mol. Catalysis A: Chemical v.101 Alcantara, A.R.;Gil, M.H.;Guiomar, A.J.;Belmonte, M.T.L.;Sobral, M.C.M.;Torres, C.;Sinisterra, J.V.
  4. Int. J. Pharm. v.131 Aydin, Z.;Akbuga, J.
  5. Process Biochemistry v.32 no.3 Siso, M.I.G.;Lang, E.;Carreno-Gomez, B.;Becerra, M.;Espinar, F.O.;Mendez, J.B.
  6. Liposome Technology(2nd ed.) v.1 Ceve, G.;Gregoriadis, G.(ed.)
  7. Liposome Ostro, M.J.
  8. Principles of Biochemistry(2nd ed.) Lehninger, A.L.;Nelson, D.L.;Cox, M.M.
  9. Liposome Technology(2nd ed.) v.1 Florence, A.T.;Gregoriadis, G.(ed.)
  10. Quarterly Review of Biophysics v.13 no.2 Israelachvili, J.N.;Marcelja, S.;Horn, R.G.
  11. J. Phy. Chem. v.76 no.21 Tanford, C.
  12. The Liposomes Nojima, S.;Sunamoto, J.;Inoue, K.
  13. Liposome Technology(2nd ed.) v.21 Gregoriadis, G.
  14. Chem. Pharm. Bull. v.17 Tsuji, A.;Kinoshita, T.;Hoshino, M.
  15. Anal. Chem. v.33 Sawicki, E.;Hauser, H.R.;Stanley, T.W.;Elbert, W.
  16. J. Kor. Ind & Eng. Chem. v.8 no.3 Lee, O.S.;Ha, B.J.;Kim, J.O.;Park, S.K.;Lee, Y.S.