Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Effects of Deamido-$\textrm{NAD}^{+}$ on Self-splicing of Primary Transcripts of Phage T4 Thymidylate Synthase Gene

  • Park, In Kook (Department of Biology, College of Sciences, Dongguk University)
  • Published : 2000.06.01
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Abstract

Effects of deamido-$\textrm{NAD}^{+}$on self-splicing of primary transcripts of the phage T4 thymidylate synthase gene (td) was investigated. The self-splicing was not affected by deamido-$\textrm{NAD}^{+}$- at concentrations up to 2 mM. However, it began to decrease at 5 mM and the formation of splicing products such as the linear intron, intron-exon 2 and exon 1-exon 2, was slightly reduced. At 20 mM the self-splicing activity was almost completely abolished. This analog of the coenzyme $\textrm{NAD}^{+}$- inhibits the self-splicing of td intron RNA although it does not possess a guanidine group in its structure. The analysis of inhibitory concentrations and structural examination suggests that the key structural features of deamido-$\textrm{NAD}^{+}$ responsible for the inhibition of splicing may be the ADP-ribose moiety.

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References

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