Journal of Microbiology
- Volume 38 Issue 4
- /
- Pages.260-264
- /
- 2000
- /
- 1225-8873(pISSN)
- /
- 1976-3794(eISSN)
Detection of Marine Birnavirus (MBV) from Rockfish Sebastes schlegeli Using Reverse Transcription and Nested PCR
- Joh, Seong-Joon (Laboratory of Aquatic Animal Disease, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
- Kim, Doo-Won (Laboratory of Aquatic Animal Disease, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
- Kim, Jeong-Ho (Laboratory of Aquatic Animal Disease, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
-
Heo, Gang-Joon
(Laboratory of Aquatic Animal Disease, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
- Published : 2000.12.01
Abstract
Reverse transcription (RT)-PCR and nested PCR methods (2-step PCR) were tested for their ability to detect marine birnavirus (MBV) in cultured rockfish, Sebastes schlegeli. One set of primers for RT-PCR was designed, based on a gene of infectious pancreatic necrosis virus (IPNV), and another set of primers for nested PCR was designed based on the VP2/NS junction region of MBV. This 2-step PCR method was specific for MBV and sensitivity was heightened when nested PCR was combined to RT-PCR. This 2-step PCR method was useful for detecting MBV not only in diseased fish, but also in asymptomatic fish. These results indicate that this 2-step PCR method is useful for detecting MBV in rockfish.