Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Genetic Diversity of Korean Cyanobacteria determined by DNA polymorphisms within the Phycocyanin Locus

Phycocyanin locus내의 DNA Polymorphism에 의한 한국산 Cyanobacteria의 유전적 다양성

  • Published : 2000.12.01
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Abstract

The genetic diversity among Korean cyanobacteria was assessed by restriction fragment length polymorphism(RFLP) analysis of PCR products from the phycocyanin locus. Strains of all the genera tested were successfully amplified, and the size of amplified fragments was approximately 700bp. The restriction patterns generated by AluI, MspI, and HaeIII were conserved for strains within each of genera studied and were specific to the genus level. Intrageneric delineation of strains was revealed by the enzyme, CfoI for members of genera Anabeana and Synechocystis. Phenogram derived from the different RFLP patterns revealed a coherent cluster among Anabeana, Chlorogloea, and Synechocystis strains. PC-RFLP methods provided useful tools for classification of cyanobacteria.

Cyanobacteria의 광합성 보조색소인 phycocyanin의 PC operon(cpc gene)을 PCR로 증폭하고, 제한효소로 처리하여 RFLP pattern을 비교하였다. Intergenic spacer sequence를 포함한 cpc gene은 실험에 사용한 cyanobacteria 균주 모두에게 증폭되었으며, 산물의 size는 약 700 bp였다. PCR산물을 5종의 제한효소로 처리한 결과 AluI, MspI, HaeIII는 같은 속내으ㅐ 균주간에 동일한 pattern을 나타내어 속 구분이 가능하였으며 CfoI은 Anabeana와 Synechocystis속의 균주간에 구별되는 양상을 나타내어 속내 균주 구별에 유용하였다. Restriction enzyme profile에 의한 phenogram에서 Anabeana, Chlorogloea, Synechyhocystis는 각각 하나의 cluster를 형성하여 cyanobacteria의 분류에 PC-IGS의 RFLP pattern이 유용함을 알 수 있었다.

Keywords

References

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