한국수정란이식학회지 (Journal of Embryo Transfer)
- 제15권3호
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- Pages.231-236
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- 2000
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
무혈청배지에 첨가된 성선자극호르몬 및 항산화제가 소 미성숙난자의 체외성숙능에 미치는 영향
Effects of Addition of Exogenous Gonadotropins and/or an Antioxidant to Serum-Free Medium on in vitro Maturation of Bovine Immature Oocytes
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임정묵
(서울대학교 농업생명과학대학 농생명공학부) ;
- 박성은 (포천중문의과대학교 의학과) ;
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정형민
(포천중문의과대학교 의학과) ;
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이병천
(서울대학교 수의과대학) ;
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이은송
(강원대학교 수의학과) ;
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고정재
(포천중문의과대학교 의학과) ;
- 박찬 (포천중문의과대학교 의학과) ;
- 차광렬 (포천중문의과대학교 의학과) ;
- 황우석 (서울대학교 수의과대학)
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Lim, J. M.
(Department of Agricultural Biotechnolgoy, College of Agriculture and Life Science, Seoul National University) ;
- Park, S. E. (College of Medicine, Pochon CHA University) ;
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Chung, H. M.
(College of Medicine, Pochon CHA University) ;
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Lee, B. C.
(College of Veterinary Medicine, Seoul National University) ;
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Lee, E. S.
(Dept. of Veterinary Medicne, Kangwon National University) ;
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Ko, J. J.
(College of Medicine, Pochon CHA University) ;
- Park, C. (College of Medicine, Pochon CHA University) ;
- Cha, K. Y. (College of Medicine, Pochon CHA University) ;
- Hwang, W. S. (College of Veterinary Medicine, Seoul National University)
- 발행 : 2000.12.01
초록
This study was conducted to examine the effects of exogenous gonadotropins (PMSG+hCG) and an antioxidant (cysteine) on in vitro maturation of bovine follicular oocytes. Cumulus-oocyte complexes (COCs) aspirated from 2 to 5 mm ovarian follicles were cultured for 22 to 24 hours in a modified bovine embryo culture medium (mBECM) supplemented with 3 mg/mL bovine serum albumin, to which PMSG (10 IU/mL) + hCG (10 IU/mL) and/or cysteine (0.6 mM) were added. When examined the expansion of cumulus ce1ls at the end of maturation culture, greater (p<0.05) expansion was found after addition of PMSG+hCG (79 to 96%) to mBECM than after no addition (0%), regardless of the presence or absence of cysteine in the medium. The addition of cysteine did not stimulate cumulus expansion, but a high proportion (92%) of expansion was achieved when COCs were cultured after the addition of PMSG+hCG and cysteine to the medium. No difference in the proportion of oocytes underwent germinal vesicle breakdown (initiation of maturation) was found after the addition of PMSG+hCG and/or cysteine to mBECM. However, nuclear maturation (development to the metaphase-II stage) of oocytes was significantly stimulated by the combined addition of PMSG+hCG and cysteine, compared with no addition. In conclusion, both exogenous gonadotropins and an antioxidant are important for nuclear maturation of bovine immature oocytes and these factors have a cell-specific stimulatory action.