Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 15 Issue 3
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- Pages.211-218
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- 2000
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Effect of Cysteine and Glutathione on Motility Index and Morphology in Human Spermatozoa
Cysteine 및 Glutathione이 사람정자의 운동성지수와 정자형태에 미치는 영향
Abstract
This experiment was conducted to investigate the effect of the cysteine and glutathione on the motility index and morphology of human spermatozoa at the sperm processing in vitro. After treating the sperm with medium containing cysteine and glutathione, we measured the motility index and morphology at 0.5 h and 24 h. 1. Following the sperm culture for 0.5 h after treating the sperm with the medium containing 0, 1, 5, 10 mM cysteine, curvilinear velocity (VCL) was significantly (p<0.05) higher in control than that in all treatments. And straight-line velocity (VSL) was high at 1 mM and average path velocity (VAP) was low at 5 mM and 10 mM. But the motility (MOT) and morphology (NOM) were not different between control and all treatments. Following the sperm culture for 24 h, the MOT was significantly high in treatment groups (58.9, 74.4 and 62.3%), compared with that in control(28.7%) and the VCL was also high in treatment groups (31.4, 37.9, and 34.0