Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of the Galactose Mutarotase Gene from Lactococcus lactis ssp. lactis ATCC7962 in Escherichia coli

  • Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Choi, Jae-Yeon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Lee, Jung-Min (Department of Food Science and Technology, Seoul National University) ;
  • Kim, Jeong-Hwan (Department of Food Science and Technology, Gyeongsang National University) ;
  • Chang, Hae-Choon (Department of Food and Nutrition, Chosun University) ;
  • Chung, Dae-Kyun (Institute and Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, Seoul National University)
  • Published : 2000.12.01
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Abstract

The structure of gal/lac operon of Lactococcus lactis ssp. lactis ATCC7962 was partially characterized and the gene (galM) encoding galactose mutarotase was cloned together with the order; galA-galM-galK-galT. The galM was found to be 1,027 bp in length and encoded the protein of 37,609 Da calculated molecular mass. The deduced amino acid sequence showed a homology with GalM proteins from several other microorganisms. Thus, the galM gene was expressed in Escherichia coli and the product was identified as a 38 kDa protein which corresponded to the size estimated from DNA sequence. mutarotase activity of the IPTG inducedrecombinant was 2.7 times increased against that of the host strain.

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References

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