한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제28권6호
  • /
  • Pages.334-340
  • /
  • 2000
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

생물방제균 Pseduomonas fluorescens 2112의 선발과 고추역병균에 대한 항진균성 길항작용

Selection and Antifungal Activity of Antagonistic Bacterium Pseudomonas sp. 2112 against Red-Pepper Rotting Phytophthora capsici

  • 이은탁 (영남대학교 자연자원대학 응용미생물학과) ;
  • 김상달 (영남대학교 자연자원대학 응용미생물학과)
  • 발행 : 2000.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In order to select multifunctional powerful antagonistic biocontrol agent against red-pepper rotting fungi Phytophthora capsici, we isolated an indigenous antagonistic bacterium which produces antifungal substances and siderophores from a local soil of Kyongju, Korea. The isolated strain was identified as Pseudomonas fluorescens biotype F. The antibiotic produced from P. fluorescens 2112 inhibited hyphae growth and the zoospore germination of Phytophthora capsici. The favorable carbon, nitrogen source and salts for the production of antibiotic from P. fluorescens 2112 were glycerol, beef extract and LiCi at 1.0%, 0.5% and 5 mM, respectively. And antagonistic activity of P. fluorescens 2112 was confirmed against P. capsici in vivo.

키워드

참고문헌

  1. Agric. Biol. Chem. v.28 Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas Arima, K.;H. Imanaka;M. Kousaka;A. Fukuta;G. Tamura
  2. J. Biol. Chem. v.118 Colorimetic determination of the components of 3,4 dihydroxyphenyalanine-tyrosine mixtures Arnow, L. E.
  3. Nature. v.167 Uptake of antibotic metabolites of soil microorganisms by plant Brian, P. W.;J. M. Wright;J. Stubbs;A. M. Way.
  4. Acta Chem. Scand. v.2 On the estimation of bound hydroxylamine Csaky, T.
  5. Am. J. Botany. v.9 Antibiotics as agents for the control of certain damping-off fungi Gregory, K. F.;O. N. Allen;A. J. Riker;W. H. Peterson.
  6. Bergey's Manual of Determinative Bacteriology. 9th Holt, J. G.;N. R. Krieg;P. H. A. Sneath;J. T. Staley;S. T. Williams.
  7. J. Microbiol. Biotech. v.4 Antifungal mechaism and properties of antibiotic substances produced by Bacillus subtilis YB-70 as a biocontrol agent Kim, Y. S.;S. D. Kim
  8. The report of reserch for the development of agricultural technology Biological control of phytopathogenic fungi by the genetic breeding of indigenous antagonistic microorganisms Lee, E. T.;S. D. Kim
  9. Agri. Chem. Biotechnol. v.42 Urification and characterization of antifungal chitinas from indigenous antagonistic Microorganism Serratia sp. 3905 Lee E. T.;S. D. Kim
  10. Agri. Chem. Biotechnol. v.42 Isolation and antifungal activity of the chitinase producing bacterium Serratia sp.3905 as Antagonistic Bacterium against Fusarium sp. Lee E. T.;S. D. Kim
  11. J. Phytopathol v.115 Antifungal effects of bacilysin and fengymycin from Bacillus subtilis F-29-3: a comparison with activaties of other Bacillus antibiotics Leoffler, W.;J.S.M. Tschen;N. Vanittanakom;M. Kugler;E. Knorpp;T.F. Heseh;T.G. Wu
  12. Phytopathol v.81 Lack of a role for fluorescent siderophore production in the biological control of Phythium damping-off of cucumber by a strain of Pseudomona putida Paulitz, T. C.,;J. E. Loper
  13. Nature v.324 Plant chitinase are potent inhibitors of fungal growth Schlumbaum, A.;F. Mauch;U. Vogeli;T. Boker
  14. Anal. Biochem. v.160 Universal chemical assay for the detection and determination of siderophores, Scgwyn, B.;J. B. Neilands
  15. J. Antibiot. v.11 Blasticidin S, a new antibiotic Takeuchi, S.;K. Hirayama;K. Ueda;H. Sasaki;H. Yonehara