Production of Compaction from Penicillium sp. Y-8515

Penicillium sp. Y-8515에 의한 Compactin 생산

  • 박주웅 (영진약품공업(주) 중앙연구소) ;
  • 이주경 (영진약품공업(주) 중앙연구소) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 박용일 (생명공학연구소(KRIBB)) ;
  • 강상모 (건국대학교 미생물공학과)
  • Published : 2000.10.01

Abstract

A strain producing high levels of compaction was isolated from soil and identified as Penicillium sp. Y-8515 based on the morphological characteristics and ribosomal RNA sequence analysis. Optimization of several different carbon and nitrogen sources for the effective production of compaction was performed resulting in the medium compositions containing 5%(w/v) glucose, 1.0 % soybean meal, 0.5% yeast extract, 0.5%(NH$_4$)$_2$$SO_4$, 0.25%,$ NaH_2$$PO_4$, 0.25% $CaCO_3$. The fixed con-centration of glucose(5%, w/v) and relatively lower concentrations(less than 2.5%, w/v) of soybean meal stimu-lated the transformation of the growth morphology from filamentous to pellet form. Comparing to that by filamentous form, the production of compactin by pellet form increased up to 1.5 folds. In a fed-batch fermentation, continuous feeding of the mixture of glucose and nitrogen source at the ratio of 10:1 showed 3.5-fold more produc-tion yield of compaction comparing to the batch mode.

Keywords

References

  1. Proc. Natl. Acad. Sci. v.77 Mevinolin A high potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent Alberts, A. W.;J. Chen;G. Kuron;V. Hunt;J. Huff;C. Hoffman;J. Rothrock;M. Lopez;H. Joshua;E. Harris;A. Patchettt;R. Monaghan;S. Currie;E. Stapley;G. Albersschonberg;O. Hensens;J. Hirshfield;K. Hoogsteen;J. Liesch;J. Springer
  2. J. CHem. Soc. perkinl v.1976 Crystal and molecular structure of compactin, a new antifungal metabolite from Penicillium brevicompactin Brown, A. G.;T. C. Samale;R. H. King;R. H. Thompson
  3. Topic in Industrial Microbiology Novel Microbial Products for Medicine and Agriculture Buckland, B.;K. Gbewonyo;T. Hallada;L. Kaplan;P. Masurekar;Demain A.(ed.);Somkuti G.(ed.);Hunger-Cevera J.(ed.);Rossmoore H.W.(ed.)
  4. Biotechnol. Bioeng. v.24 Strategies for penicillin fermentation in Tower loop reactors Burghard, K.;K. Schugerl
  5. The Compendium of Soil Fungi Domsch, K. H.;W. Gams;T. H. Anderson
  6. J. Antbiot. v.29 ML-236A, ML-236B and ML-236C, new inhibitors of cholesterogenesis produced by Penicillium citrinum Endo, A.;M. Kuroda;Y. Tsujita
  7. J. Antibiot. v.29 Citrinin, an inhibitor of cholesterol synthesis Endo A.;M. Kuroda
  8. J. Antibiot. v.38 Biosynthesis of ML-236B(compactin) and Monacolin K Endo, A.;N. Yoshinori;I. Takashi;M. Kosei;H. Masahiro
  9. J. Antibiot. v.39 The synthesis of compactin(ML-236B) and monacolin K in fungi Endo, A.;H. Keiji;Y. Akira;S. Rika;T. Hiroshi
  10. J. Lipid Reseach. v.33 The discovery and development of HMG-CoA reductase inhibitors Endo, A.
  11. J. Med. Chem. v.29 2-hydroxy-3-methylglutaryl-coenzymc A reductase inhibitor. 4 side chain ester derivatives of mevinolin Hoffmen, W. F.;A. W. Alberts;P. S. Anderson;J. S. Chen;R. L. Smith;A. K. Willard
  12. Biosci. Biotech. Biochem. v.57 Production of ML-236B, and inhibitor of 2-hydorxy-3-methyglutaryl Co A redutase, by Penicillium citrinum : Improvements of strain and culture conditions Hosobuchi, M.;S. Testsuya; O. J.;A. Masatoshi;I. Seigo;Y. Hiroji
  13. J. Ferment. Bioeng. v.76 Morphology control of preculture during production of ML-236B, a precursor of pravastatin sodium, by Penicillium citrinum Hosobuchi, M.;F. Fukui;H. Matsukawa;T. Suzuki;H. Yoshikawa
  14. J. Ferment. Bioeng. v.76 Morphology control of preculture during production of ML-236B, a precursor of pravastatin sodium, by Penicillium citrinum Hosobuch, M.;F. Fukui;H. Matsukawa;T. Suzuki;H. Yoshikawa
  15. A manual of Soil fungi(2nd) Joseph C. Gilman
  16. J. Bio Chem. v.234 The biosynesis of mevalonic acid from$1-^{14}$C-acetate by rat liver enzyme system Kanuss, H. J.;J. W. Rorter;G. Wasson
  17. Anal. Chem. v.31 Use of dinitrosalicylic acid regent for determination of reducing Sugar Miller, G. L.
  18. Mycologia v.87 Phylogenetic relationships in Ganod er ma nferred from the internal transcribed spacers and 25S ribosomal DNA sequences Moncalvo, J. M.;H. H. Wang;R. S. Hseu
  19. Biotechnol. Bioeng. v.35 Morphology and physiology of an α-amylase producing strain of Aspergillus oryzae during batch cultivations Morten, C.;A. B. Spohr;J. Nielsen;J. Villadsen
  20. J. Ferment. Bioeng. v.76 Fuzzy control in microbial production of ML-236B, a procursor of pravastatin sodium Masahiko, H.;F. Fukui;T. Suzuki;H. Yoshikawa
  21. J. Antibiot. v.36 Microbial hydroxylation of ML-236B(compactin) and monacolin A(MB-530B) Nobufusa, S.;N. Keiko;H. Kiyoshio;T. Yoshio;T. Akira;K. Harumitsu
  22. Biotechnol. Bioeng. v.35 Rhe effect of agitation on the morphology and penicillin production of Penicillium chrysogenum Smith, J. J.;M. D. Lilly;R. I. Fox
  23. Atherosclerosis v.32 Hypolipidemic effects in dogs of ML-236B, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reduetase Tsujia, Y.;K. Tanzaw;N. Kitano;A. Endo
  24. Biotech. Bioeng. v.33 Effect of nutriion on pellet formation by Rhizopus arrhizus Ward, O. P.;G. S. Byrne
  25. PCR Protocols: A guide to methods and application Amplification and direct sequencing of fungal ribosornal RNA fenes for phylogenetics White, T. J.;T. Bruns;S. Lee;J .W. Taylor;Innis, M.S.(ed.);D.H. Gelfand(ed.);J.J. Sninsky(ed.);R.J. White(ed.)