Effect and Development of Automatic Control of Dissolved Oxygen on Growth of Phellinus linteus WI-001

Phellinus linteus WI-001 균사체의 성장에 미치는 용존산소농도의 영향 및 자동 조절방법 개발

  • 김종래 (환인제약(주) 중앙연구소,강원대학교 자연과학대학 생명과학부) ;
  • 권호균 (환인제약(주) 중앙연구소) ;
  • 전계택 (강원대학교 자연과학대학 생명과학부) ;
  • 이계관 (환인제약(주) 중앙연구소)
  • Published : 2000.10.01

Abstract

The effects of dissolved oxy-gen(DO) concentration and DO control modes on cell growth in a 4L computer-controlled bioreactor system were investigated using Phellinus linteus WI-001, a producer of protein-polysaccharides having potent anticancer activi-ties. When DO was controlled at about 20%, maximum cell concentration and specific growth rate of the strain were observed to increase 36% and 64%, respectively, as compared to the experiment performed without DO control. By adopting cascade automatic control of DO ar 20% in a mixed automatic control mode using computer-con-trolled program, 19.5g/L of maximum cell concentration was obtained. These results showed that the mixed auto-matic control mode was the effective method for enhancing cell growth of the shear sensitive Phellinus linteus.

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References

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