Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparison of Promoters Suitable for Regulated Overexpression of $\beta$-Galactosidase in the Alkane-Utilizing Yeast Yarrowia lipolytica

  • Thomas Juretzek (Institut fur Mikrobiologie, Technische Universitat Dresden) ;
  • Hui-Jie wang (Laboratoire de Microbiologie et de Genetique Moleculaire et Cellulaire INRA Centre de Grignon) ;
  • Nicaud, Jean-Marc (Laboratoire de Microbiologie et de Genetique Moleculaire et Cellulaire INRA Centre de Grignon) ;
  • Stephan Mauersberger (Institut fur Mikrobiologie, Technische Universitat Dresden) ;
  • Gerold Barth (Institut fur Mikrobiologie, Technische Universitat Dresden)
  • Published : 2000.09.01
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Abstract

Promoters of the genes G3P, ICL1, POT1, POX1, POX2 and POX5 of the yeast Y. lipolytica were studied in respect to their regulations and activities during growth on different carbon sources. The aim of this study was to select suitable promoters for high expression of heterologous genes in this yeast. For this purpose the promoters were fused with the reporter gene lacZ of E. coli and integrated as single copies into the genome of Y. lipolytica strain PO1d. The measurement of expressed activities of ${\beta}$-galactosidase revealed that pICL1, pPOX2 and pPOT1 are the strongest regulable promoters available for Y. lipolytica, at present. pPOX2 and pPOT1 were highly induced during growth on oleic acid and were completely repressed by glucose and glycerol. pICL1 was strongly inducible by ethanol besides alkanes and fatty acids, however, not completely repressible by glucose or glycerol. Ricinoleic acid methyl ester appeared as a very strong inducer for pPOT1 and pPOX2, in spite of that it inhibited growth of Y. lipolytica transformants.

Keywords

References

  1. Yarrowia lipolytica Nonconventional yeasts in biotechnology Barth, G.;C. Gaillardin
  2. FEMS Microbiol. Rev v.19 Physiology and genetics of the dimorphic fungus Yarrowia lipolytica Barth, G.;C. Gallardin
  3. J. Basic Microbiol v.28 Genetic engineering in Yarrowia lipolytica Gaillardin, C.;H. Heslot
  4. Yeast v.8 Foreign gene expression in yeast Romanos, m.;C. Scorer;J. Clare
  5. Yeast v.10 Non-Saccharomyces Yeasts Sydbery, P
  6. Yeast v.14 Comparison of expression systems in the yeasts Saccharomuces cerevisiae, Hansenula polymorpha, Klyuveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica Muller, S.;T. Sandal;P. Kamp-Hansen;H. Dalboge
  7. Yeast v.7 Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica Fournier, P.;L. Guyaneus;M. Chasles;C. Gaillardin
  8. Proc. Natl. Acad. Sci USA v.90 Colocalization of centromeric and replicative fuctions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica Fournier, P.'A. Abbas;M. Chasles;B. Kudla;D. M. Ogrydziak;D. Yaver;J. -W. Xuan;A. Peito;A. -M. Ribet;C. Feunerol;F. He;C. Gaillardin
  9. Mol. Gen. Genet v.237 Analysis of regions essential for the fuction of chromosomal replicator sequences from Yarrowia lipolytica Matsuoka, M.;M. Matsubara;H. Daidoh;T. Imanaka;K. Uchida;S. Aiba
  10. Mol. Cell. Biol. v.17 An origin of replicarion and a centromere are both needed to setablish a replicative plasmid in the yeast Yarrowia lipolytica Vernis, L.;A. Abbas;M. Chasles;C. M. Gaillardin;C. Brun;J. A. Huberman;P. Fourmier
  11. Mol. Cell. Biol. v.10 Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica Vernes, L.;M. Chasles;P. Pasero;A. Lepingle;C. Gaillardin;P. Fournier
  12. Curr. Genet v.26 Multi-copy integration in the yeast Yarrowia lipolytica Le Dall. M. -T.;J.-M. Nicaud;C. Gaillardin
  13. Vectors for gene expression and amplification in the yeast Yarrowia lipolytica Juretzet, T.;M. T. Le Dall;S. Mauersberger;C. Gaillardin;G. Barth;J. M. Nicaud
  14. Mol. Cell. Biol. v.8 Intracellular precursors and secretion of alkaline extracellular protease of Yarrowia lipolytica Matoba, S.;J. Fukuyama, R. A. Wing;D. M. Ogrydziak
  15. J. Biol. Chem. v.266 Role of the proregion in the production and secretion of the Yarrowia lipolytica alkaline extracellular protease Fabre, E.;J. M. Nicaud;M. C. Lopez;C. Gaillardin
  16. Microbiology v.143 Dipeptidyl aminopeptidase processing and biosynthesis of extracellular protease from Yarrowia lipolytica Motoba, S.;K. A. Morano;D. J. Klionsky;K. Kim;D. M. Ogrydziak
  17. Mol. Cell. Biol. v.14 Two upstream UAS control expression of the XPR2 gene encoding an extracellular alkaline prodease in the yeast Yarrowia lipolytica Blanchin-Roland, S.;R. Cordero Otero;D. Gaillardin
  18. J.Biol. Chem v.272 Expression, secretion, and processing of rice a-amylase in the yeast Yarrowia lipolytica Park, C. S.;C. C. Chang;J. Y. Kim;D. M. Ogrydziak;D. D. Ryu
  19. Biotechnol. Bioeng v.59 Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed-batch process strategy Chang, C. C.;D. D. Ryu;C. S. Park;J. Y. Kim
  20. Microbiology v.145 Fuctional analysis of upstream regulating regions from the Yarrowia lipolytica XPR2 prompter Madzak, C.;S. Blanchin-Roland;P. R. Cordero-Otero;C. Gaillardin
  21. Mol. Gen. Genet v.241 Cloning of the isocitrate lyase gene(ICL1) from Yarrowia lipolytica and characterization of the deduced protein Barth, G.;T. Scheuber
  22. Eur. J. Biochem v.216 Structure and metabolic control of the Yarrowia lipolytica peroxisomal 3-oxozcyl-CoA-thiolase gene Berninger, G.;R. Schmidtchen;G. Casel;A. Knoerr;K. Rautenstrauss;W.-H. Kunau;E. Schweizer
  23. Yearst v.14 Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane-utilizing yeast Yarrowia lipolytica Wang, H.;A. Le Clainche;M. T. Le dall;Y. Wache;Y. Pagot;J. M. Belin;C. Gaillardin;J. M. Nicaud
  24. J. Bacteriol v.181 Evalution of acyl coenzyme A oxidase (Aox) isozyme fuction in the n-alkane-assimilating yeast Yarrowia lipolytica Wang, H. J.;M. T. Le Dall;Y. Wach;C. Laroche;J. M.Belin;C. Gaillardin;J. M. Nicaud
  25. A laboratory manual(2nd ed) Molecular cloning Sambrook, J.;E. F. Fritsch;T. Maniatis
  26. Candida maltosa Nonconventional yeases in biotechnology Mauersberger, S.;M. Ohkuma;W.-H. Schunck;M. Takagi;K. Wolf(ed.)
  27. Current Protocols in Molecular Biology v.1 Ausubel, F. M.;R. Brent;R. E. Kingston;D. D. Moore;J. G. Seidman;J. A. Smith;K. Struhl
  28. Entwicklung von Wirts-Vektor-Systemen zur heterologen Expression von Proteinen in der nichtkonventionellen Hefe Yarrowia lipolytica und ihre Anwendung fur die Cytochrom P450-katalysierte Stoffumwandlung Juretzet, T.
  29. Untersuckungen zur Regulation des 3-Oxoacyl-CoA-Thio-lase-Ⅰ-Gens aus Yarrowia lipolytica Schmidtchen, R.
  30. J. Mol. Microbiol. Biotechnol. v.2 Strong hybrid prompters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica Madzak, C.;B. Treton;S. Blanchin-Roland
  31. Proc. Netl. Acad. Sci. USA v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;S. Nicklen;A. R. Coilson
  32. Curr. Genet v.11 LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrowia lipolytica Gaillardin, C.;A.-M. Ribet
  33. Lett. Appl. Microbiol. v.25 Utilization of an auxotrophic strain of the yeast Yarrowia lipolytica to improve ganna-decalactone production yields Pagot, Y.;A. Endrizzi;J. M. Nicaud;J. M. Belin
  34. Untersuchungen zur Optimierung des Elektronentransportsystens fur die Cytochrom P450 katalysierte Biotransformation von Steroiden in Yarrowia lipolytica Gerber, J.