Abstract
Time course monitoring of FCW/DCW ratio, production of intra and extracellular protein, and peroxidase activity were performed during suspension culture of Taxus chinensis cells. The observed FCW/DCW ratio was 12 at day 14, which was the lowest value during cultivation, and the specific protein production, based on dry cell weight, was also the lowest at day 14, which showed 4.3 mg/g DCW. The pattern of POD activity was similar to that of protein production. The results in this report were obtained using actively growing cells in flasks, therefore it is possible to use those results to control the process and indicate the stresses imposed on cells during large-scale cultivation.
Taxus chinensis 세포배양 중의 FCW/DCW ratio, 세포내, 외 단백질 생산 및 peroxidase 활성의 변화를 배양시기별로 조사하였다. 조사 결과, 배지내 잔류당이 고갈되기 직전인 배양 14일째에 FCW/DCW ratio 는 12로 가장 낮은 수치를 나타내었고 DCW를 기준으로 한 세포 외에서의 protein 생산도 14일째 가장 낮은 수치인 4.3 mgfg DCW 였다. 배양시기별 P POD 활성도는 단백질 생산 pattern과 유사하였다. 이 결과들 은 flask에서 활발하게 증식 중인 세포들을 이용하여 측정한 것으로 대량배양시, 공정제어를 위한 자료 및 세포가 받는 stress 정도를 나타내는 지표로 활용할 수 있다
