Effects of Growth Substrates on Cometabolic Biodegradation of Trichloroethylene by Burkholderia cepacia G4

Burkholderia cepacia G4에 의한 트리클로로에틸렌의 공동대사적 분해에 미치는 성장기질의 영향

  • 예병대 (부산대학교 공과대학 화학공학과) ;
  • 박성훈 (부산대학교 공과대학 화학공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • Published : 2000.10.01

Abstract

The effects of growth substrates such as toluene and phenol on cometabolic biodegradation of trichloroethylene (TCE) by Burkholderia cepacia G4 were investigated. The dual effects of primary substrate on TCE biodegradation, stimulatory effects of toluene and phenol at low concentrations (0.5∼2 ppm & 0.1∼0.5 ppm, respectively) and a competitive inhibition at high concentration, were observed in batch experiments. These stimulatory effects of toluene and phenol were found to be due to the increments in the amount of reducing power like NADH which could be generated during the assimilation of toluene and phenol as the carbon and energy source. The efficiency of TCE biodegradation in trickling biofilm reactor (TBR) could be also enhanced up to the TCE removal efficiency of 58.1% by the supply of appropriate amounts of phenol (0.94∼4.7 ppm).

Keywords

References

  1. Annu. Rev. Microbiol. v.45 Biochemical diversity of trichloroethylene metabolism Ensley, B. D.
  2. J. Am. Water Works Assoc. v.5 The groundwater supply survey Westrick, J. J.;J. W. Mello;R. F. Thomas
  3. Appl. Environ. Microbiol. v.55 Survey of microbial oxygenases: Trichloroethylene degradation by propane-oxidizing bacteria Wackett, L. P.;G. A. Brusseau;S. R. Householder;R. S. Hanson
  4. Appl. Environ. Microbiol. v.57 Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichlorosporium OB3b and toxicity of trichloroethylene Oldenhuis, R.;J. Y. Oedzes;J. J. van der Waarde;D. B. Janssen
  5. Reviews of Environmental Contamination and Toxicology Trichloroethylene: Water contamination and health risk assessment Fan, A. M.;G.W. Ware(ed.)
  6. Proposed. Fed. Regist. v.49 National Primary Drinking Water Standard U. S. EPA
  7. Appl. Environ. Microbiol. v.57 Effects of toxicity, aeration, and reductant supply on trichloroethylene transformation by a mixed methanotrophic culture Alvarez-Cohen, L.;P. L. McCarty
  8. Appl. Environ. Microbiol. v.55 Degradation of Chlorinated Aliphatic Hydrocarbons by Methlosinus thrichosporium OB3b Expressing Soluble Methane Monooxygenase Oldenhuis;R. L. vink;D. B. Janssen;B. Withholt
  9. Biotechnol. Bioeng. v.38 Batch cultivation of Methylosinus trichosporium OB3b. Ⅰ: Production of soluble methane monooxygenase Park, S.;M. L. Hanna;R. T. Taylor;M. W. Droege
  10. Appl. Environ. Microbiol. v.55 Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b Tsien, H. C.;G. A. Brusseau;R. S. Hanson;L. P. Wackett
  11. Environ. Sci. Technol. v.29 Model for the cometabolic biodegradation of chlorinated organics Chang, H. L.;L. Alvarez-Cohen
  12. Appl. Environ. Microbiol. v.55 Toxicity of trichloroethylene to Pseudomonas putida F1 is mediated by toluene dioxygenase Wackett, L. P.;S. R. Householder
  13. Appl. Environ. Microbiol. v.57 Performance Characterization of a Model Bioreactor for the Biodegradation of Trichloroethylene by Pseudomonas cepacia G4 Folsom, B. R.;P. J. Chapman
  14. Appl.-Environ. Microbiol. v.53 Trichloroethylene Metabolism by Microorganisms that Degrade Aromatic Compounds Nelson, M. J.;S. O. Montgomery;W. R. Mahaffey;P. H. Pritchard
  15. Environ. Sci. Technol. v.31 Modeling of the Cometabolic Biodegradation of Trichloroethylene by Toluene-Oxidizing Bacteria in a Biofilm System Jean, P. A.;E. Arvin
  16. Biotechnol. Bioeng. v.69 Kinetic Analysis of a tod-lux Bacterial Reporter for Toluene Degradation and Trichloroethylene Cometabolism Kelly, C. J.;P. R. Bienkowski;G. S. Sayler
  17. In: Molecular cloning: a laboratory manual Maniatis T.;Fritsch EF.;J. Sambrook
  18. Appl. Microbiol. Biotechnol. v.45 Evaluation of trichloroethylene degradation and mineralization by pseudomonads and Mehtylosinus trichosporium OB3b Sun, A. K.;T. K. Wood
  19. M.S. Thesis, Dept. of Chemical Eng. Degradation of ethylene chlorides by Methylosinus trichosporium Kim, K. T.
  20. Appl. Environ. Microbiol. v.56 Phenol and trichloroethylene degradation by Pseudomonas cepacia G4; Kinetics and Interactions between substrates Folsom, B. R.;P. J. Chapman;P. H. Pritchard
  21. Environ. Sci. Technol. v.31 Long-Term Biodegradation of Trichloroethylene Influenced by Bioaugmentation and Dissolved Oxygen in Aquifer Microcosms Junko M. M.;V. G. Matheson;L. J. Forney;J. M. Tiedje;P. L. Mccarty
  22. HWAHAK KONGHAK v.57 Degradation Kinetics of Trichloroethylene by Methylosinus trichosporium Kim, K. T.;J. W. Hwang;J. M. Kang;S. Park