Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Vitamin E Supplementation on Renal Lipid Peroxidation in High Fat Diet and Adriamycin Induced Experimental Nephrotic Syndrome Model Rats

고지방식이와 Adriamycin으로 유도된 신증후군 흰쥐실험모델에 비타민 E 첨가식이가 신장의 지질과산화대사에 미치는 영향

  • 박영주 (서울대학교 생활과학대학 식품영양학과)
  • Published : 2000.03.01
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Abstract

This study was conducted to investigate the effects of vitamin E supplementation renal lipid peroxidation in high fat diet and adriamycin (ADR) induced experimental nephrotic syndrome model rats. Treated rats were injected intraperitoneally with ADR (2mg/kgBW/wk) once a week for four weeks. control rats were injected with saline solution instead of ADR. The rats in each group were fed experimental diets of three levels of vitamin E for 10 weeks: Normal (501U/kg diet), high (5,000IU/kg diet), excess (7,500IU/kg diet). The high fat diet and ADR treatment was performed to induce the decrease of kidney functions. Serum total cholesterol was significantly decreased by the excess supplementation. But there was no effect of vitamin E supplementation on serum total lipid and triglyceride. Thiobarbituric acid reacting substances(TBARS) was significantly decreased at high and excess supplementation. Glutathione reductase (GR), glutathione peroxidase ({TEX}$GP_{x}${/TEX}) and catalase activities (CAT) were measured as antioxidative enzymes. The renalglutathione reductase (GR) and catalase activities (CAT) were inclined to elevate by vitamin E supplementation. Thus the vitamin E supplementation was found to have an antioxicant effect. These results suggested that vitamin E supplementation could alleviate the changes in renal lipid peroxidation.

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References

  1. Kidney Int Suppl v.31 Hyperlipidemia and progressive renal disease Keane WF;Mulcahy WS;Kasiske BL;Kim Y;O'Donnell M
  2. Kindey Int v.37 Renal injury of diet-induced hypercholesterolemia in rats Kasiske BL;O'Donnell MP;Schmitz PG;Kim Y;Kean WF
  3. Am J Physiol v.260 Branded chain amino acids augment neither albuminemia nor albumin synthesis in nephrotic rats Kaysen GA;Al-Banden H;Martin VI;Jones H Jr;Butchison FN
  4. Am J Clin Nutr v.47 Hyperlipidemia and the pregression of renal disease Keane WF;Kasiske BL;O'Donnell MP
  5. Lancet v.2 Lipid nephrotoxicity in chronic progressive glomerural and tubulo-interstitia disease Moorehead JF;Nakos EI;Chan MK;Varghese Z
  6. national Nutrition Survey Report Ministration of Health and Welfare(1994)
  7. Histochem J v.27 Immunohistochemical localization of antioxidant enzymes during hamster kidney development Oberley TB;Sempt JM;Oberley LW
  8. J Natl Cancer Inst v.55 no.6 Adriamycin, a review Carter SK
  9. Toxicol Appl Pharmacol v.49 The effects of alpha-tocopherol on the toxicity, disposition and metabolism of adriamycin in mice Minnaugh EG;Siddik ZH;Drew. K;Sikic BI;Gram TE
  10. Toxicol v.95 Adriamycin induced hepatic and myocardial lipid peroxidation and DNA damage and enhanced excretion of urinary lipid metabolites in rats Bagchi D;Bagchi M;Hassoun EA;Kelly HJ;Stohs SJ
  11. Nephron v.60 Suppressive effect of superoxide dismutase on adriamycin nephropathy Okasora T;Takikawa T;Utsunomiya Y;Senoh I;Hayashibara H;Shiraki K;Kasagi T;Shimizu F
  12. Nephron v.68 Alpha tocopherol improves focal glomerulosclerosis in rats with adriamycin induced progressive renal failure Washio M;Nanishi F;Okuda S;Onoyama K;Fujishima M
  13. Am J Clin Nutr v.53 Protective role of Vitamin E in biological systems Packer L
  14. J Hepatol v.7 no.3 Effect of ethanol feeding and withdrawal on plasma glutathione elimination in the rat Callans DJ;Wacker LS;Mitchell MC
  15. J Am Soc Nephrol v.9 Failure of antioxidant therapy to attenuate interstitial disease in rats with reversible nephrotic syndrome Drukker A;Eddy AA
  16. Kidney Int v.51 Dietary antioxidant and renal injury in experimental focal segmental glomerulosclerosis Lee HS;Jeong JY;Kim BC;Kim YS;Zhang YZ;Chung HK
  17. Kidney Int v.53 no.5 Interstitial fibrosis in hypercholesterolemic rats: Role of oxidation, matrix synthesis and proteolytic cascades Eddy AA
  18. Kidney v.43 A multivitamin infusion prevents lipids peroxidation and improves transplant performance Rabl H;Khoschsorar G;Colombo T
  19. Am J Clin Pathol v.52 The Colorimetric method for determination of serum total lipids based on the sulfo-phosphovanillin reaction Fringe CS;Dunn RM
  20. Clin Chem v.21 A manual colorimetric assay of triglyceride in serum Biggs HG;Erikson JM;Moorhead WR
  21. Anal Biochem v.29 Study of a new cholesterol reagent Zlakis A;Zak B
  22. In Methods in enzymology v.52 Buege JA;Aust SD
  23. Methods Enzymol v.105 Spectrophotometic detection of lipid conjugated dienes Recknagel RO;Glende EA
  24. Glutathione Reductase In Methods in enzymology Carlberg I;Mannervik B
  25. Methods Enzymol v.52 Glutathione peroxidase and hyodroperoxides Tappel AL
  26. Methods of Enzymatic Analysis(end) v.2 Catalase Aebi H;.Bergmeyer, H.U.(Eds.)
  27. J Biol Chem v.193 Protein measurement with the folin phenol reagent Lowry OH;Rose brough NJ;Farr AL;Randall RT
  28. Nephron v.68 Renal impairment and intraglomerular mononuclear phagocytes in cholesterol fed rabbits Yoshimura N;Arima S;Nakayama M;Sato T;Takahashi K
  29. J Nutr v.107 Effect of high levels of dietary vitamin E on liver and plasma lipids and fat soluble vitamin in rats Jack Yang NY;Desaei ID
  30. Anal Biochem v.95 Assay for lipid peroxidation in animal tissues by thiobabituric acid reaction Ohkawa H;Ohish N;Yagi K
  31. Kidney Int v.33 Effect of dietary protein restriction on oxygen consumption and oxidant Nath KA;Croat J;Hosteler TH
  32. J Nutr v.103 Effect of dietary Vitamin E on the Activities of the Glutathione Peroxidase System in Rat Tissues Chow CK;Reddy K;Tappel AL
  33. J Nutr v.109 Influence of dietary vitamin E on MDA level in liver and adipose tissue and on glutathione peroxidase and reductase activities in liver erythrocytes of lean obese mice Trostler N;Brady PS;Romsos DR
  34. Diabetes v.36 Alterations in free radical tissue defense mechanism in streptozotocin induced diabetes in rats Wohaieb SC;Codin DV