Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Transient Expression of Transgene Introduced by Lipofected Sperm in Olive Flounder(Paralichthys olivaceus)

  • Jeong, Chang-Hwa (Research Center for Ocean Industrial Development and Dept. of Aquaclture. Pukyong National University) ;
  • Cho, Young-Sun (Research Center for Ocean Industrial Development and Dept. of Aquaclture. Pukyong National University) ;
  • Nam, Yoon-Kwon (Research Center for Ocean Industrial Development and Dept. of Aquaclture. Pukyong National University) ;
  • Park, In-Seok (Department of Marine Living Resources Kunsan National University) ;
  • Bang, In-Chul (Dept. of Aquaclture. Pukyong National University)
  • Published : 2000.03.01
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Abstract

The successful gene transfer and transient expression was demonstrated in olive flounder embryos using lipofected sperm. Olive flounder sperm interacted with foregn plasmid DNA encapsidated by positively charged liposome. The maximum plasmid copy number that associated with the sperm was 5 copies/sperm based on the examination of DNA blot assay. The foreign DNA was transferred into fertilized eggs without any adverse effect on fertilization and survival of embryos (P>0.05) and retained in embryos until at least 42 hours with successful expression. The maximal expression was detected in 18 hours after fertilization at 18$^{\cird}C$ and gradually decreased with development of embryo. Most of DNA transferred into embryos persisted extrachromosomally without significant sign of integration or replication.

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References

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