Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Tea Fungus/Kombucha Beverage on Growth, Serum Protein Level and Enzyme Activities in Male Rats

발효홍차가 흰쥐의 성장률, 혈청 단백질농도 및 효소활성에 미치는 영향

  • 고진복 (신라대학교 생명과학과)
  • Published : 2000.06.01
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Abstract

The current study was undertaken to determine the prolinged effects of fasting-refecding on the lipoprotein lipase (LPL) activity and lipogenesis of adipose tissus in male Sprague-Dawley rals and to investigate the effects of various degrecs of food restriction during refeeding on the LPL activity and lipogenesis. The control group (n=5) was fed ad libitum and killed in the fed state at the beginning of the experiment. All rats except conteol group were fasted for 2 days (n=50). Five rats were killed at the end of fasting and others (n=45) were refed either ad libitum (ad libitum group) or mildly restricted (20% food restricted group), or esverely restricted diet (40% food restricted group). Rats were killed on the day of 7th, 14th, and 21st of refeeding. Lipogenesis was determined by the amount of glucose converted to the total lipid. Body weight and epididymal adipose tissue weight returned to control states by 5 days in ad libitym group and by 14 days in 20% food restricted group. As expected, in 40% food restriction during refeeding weight and epididymal adipose tissue weight did not rcturn to control states until day 21. On day 21 after refeeding, the serum total cholesterol concentration of ad libitum group was significantly (p<0.05) higher than that of control group. The serum HDL-C concentration of 40% food restricted group during refeeding was significantly (p<0.05) higher than that of control group. However, there were no significant dif-ferences in serum HDL-C/total cholesterol (TC) ratio and trigluceride concentration among the groups. Fasting for 2 days decreased lipogenesis and LPL activity (p<0.05). On day 21 after refecding, the lipogencsis of ad libitum group was significantly (p<0.05) lower than that of control group. The lipogenesis of 40% food restricted group during refeeding was significantly (p<0.05) higher than that of conrtol group. Ad libitum group and 20% food restricted group during refecding allowed heparin-releasable (HR) LPL or total extractable (TE) LPL activity to return to control states. 40% food restricted group during refeeding delayed the return of HR-LOL or TE-LPL activity to return to control states until day 21 of refeeding. These results suggest that food restriction during refeeding can partially or completely prevent the overshoot of LPL activity, and this may influence the rate of lipid accumulation in adipose tissue during refeeding.

본 연구는 절식 및 재급여가 Sprague-Dawley계통 수컷 흰쥐(55마리)의 지방조직내 LPL활성과 지방합성량에 미치는 영향을 구명하였다. 아울러 재급여하는 동안 다양한 식이섭취 제한이 LPL활설과 지방홥성에 어떻게 영향을 미치는 지도 조사하였다. 대조군(5마리)은 무제한 식이를 급여하였으며 실험 첫날 식이섭취 상태에서 바로 희생시켰으며, 그 외 모든 쥐는 2일간 절식시켰다. 2일 절식 후 그중 5마리의 주를 희생하여 절식군으로 두었으며, 나머지 쥐(45마리)는 절식 후 식이를 재급여하였는데, 이때 재급여하는 동안 식이섭취 제한 효과를 보기 위해 adlibitum group, 20% food restricted group(ad libitum group의 80%) 및 40% food restricted group(ad libitum group의 60%)로 나누어 실시하였다. 재급여 후 7일, 14일 및 21일째 되는 날 각각 희생하였다. 지방조직의 지방함성은 포도당이 총 지방이므로 변한 양으로 측정하였다. 체중과 지방조직의 무게는 재급여 후 ad libitum group은 5일만에, 20% food restricted group은 14일만에 대조군 수준에 도달하였다. 재급여시 40% food restricted group의 체중과 지방조직의 무게는 재급여 후 21일째가 되도록 대조군 수준에 도달하지 못했다. 식이 재급여 후 21일째 ad libitum group의 혈청 총 콜레스테롤함량은 대조군에 비해 높았다(p<0.05). HDL-콜레스테롤함량은 재급여시 40% food restricted group가 대조군에 비해 높았다 (p<0.05). 그러나 HDL-C/TC비율과 중성지방함량은 처리군간에 유의적인 변화를 나타내지 않았다. 절식 후 지방조직내 지방합성량과 LPL(HR-LPL과 TE-LPL)활성은 대조군에 비해 현저히 저하되었다(p<0.05). 재급여 후 21일째 ad libitum group의 지방합성량은 대조군에 비해 낮았다(p<0.05). 그러나 40% food restricted group의 지방합성량은 대조군에 비해 높았다(p<0.05). HR-LPL과 TE-LPL활성은 재급여시ad libitum group과 20% food re-stricted group은 재급여 후 21일째가 되도록 대조군수준에 도달하지 못했다. 이러한 결과를 통해 절식 후 식이를 재급여할 경우 식이섭취 제한은 LPL활성이 증가되는 것을 부분적으로 혹은 완전히 예방할 수 있으며, 지방조직내 지방축적에 영향을 미친다는 것을 말해 준다.

Keywords

References

  1. J. Gen. Intern. Med. v.12 Probable gastrointestinal toxicity of Kombucha tea Srinivasan, R.;Smolinske, S.;Greenbaum, D.
  2. Mycologia v.57 A millennium of fungi. Food and Fermentation Hesseltine, C. W.
  3. J. Food Hyg. Society(Japan) v.13 Microorganisms of zoogloeal mats formed in tea decoction Kozaki, M.;Koizumi, A.;Kitahara, K.
  4. Dtsch. Lebensm. Rdsch. v.83 The tea fungus and its metabolic products Reiss, J.
  5. Z. Lebensm. Unters. Forsch. v.198 Influence of different sugars on the metabolism of the tea fungus Reiss, J.
  6. A study on fermentation of black tea beverage by tea fungus Choi, M. A.
  7. Biotechnology Letters v.18 Characterization of the tea fungus metabolites Blanc. P. J.
  8. Pharmaceutia Acta Helvetiae v.32 On the tea fungus Steiger, K. E.;Steinegger, E.
  9. Zentralbl. Bakt. Parasit. Inf. Hyg. v.180 Der Teepilz und seine antibiotische Wirkung Stadelman, E.
  10. Schweiz. Rundsch. Med. Prax. v.79 Sklenar's kombucha mushroom infusion a biological cancer therapy Hauser, S. P.
  11. Kombucha, healthy beverage and natural remedy from the Far Easi Frank, G. W.
  12. Acta Biotechnologica v.16 Investigation into the antibiotic activity of tea fungus/kombucha beverage Steinkraus, K. H.;Shapiro, K. B.;Hotchkiss, J. H.;Mortlock, R. P.
  13. 1995 Morbidity Mortality Weekly Report v.44 Unexplained severe illness possible associated with consumption of kombucha tea-Iowa Currier, R. W.;Goddard, J.;Buechler, K.
  14. J. Korean Soc. Food Sci. Nutr. v.28 Effect of tea fungus/kombucha on lipid metabolism in streptozotocin-induced diabetic male rats Koh, J. B.;Choi, M. A.
  15. J. Biol. Chem. v.177 Determination of serum proteins by means of the Biuret reaction Gornall, A. G.;Bardawill, C. J.;David, M. M.
  16. Clin. Chim. Acta v.37 Serum albumin estimation : modification of the bromcresol green method McPherson, I. G.;Everard, D. W.
  17. Todd-Sanford Clinical Diagnosis by Laboratory Methods. 13th ed. Davidson, I.;Henry, J. B.
  18. Technology and Clinical Pathology, 2th ed. Medical laboratory
  19. Am. J. Clin. Pathol. v.28 A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase Reitman, S.;Frankel, S.
  20. J. Clin. Pathol. v.7 Estimation of plasma phosphatase by determination of hydrolyzed phenol with aminoantipyrine Kind, P. R. N.;King, E. J.
  21. Indian J. Biochem. Biophys. v.26 Influence apices and spice principles on hepatic mixed function oxygenase system in rats Sambaiah, K.;Srinivasan, K.
  22. J. Nutr. v.103 Contribution of trypsin inhibitors to the deleterious effects of unheated soybeans fed to rats Kakade, M. L.;Hoffa, D. E.;Liener, I. E.
  23. Korea J. Lab. Ani. Sci. v.11 Reference value of hematology and serum chemistry in Ktc : Spraque-Dawley rats Kang, B. H.;Son, H. Y.;Ha, C. S.;Lee, H. S.;Song, S. W.
  24. Korean J. Lab. Ani. Sci. v.9 Effects of the population density on growth and various physiological values of Sprague-Dawley rats Kim, H. Y.;Song, S. W.;Ha, C. S.;Han, S. S.