Preventive Nutrition and Food Science
- Volume 5 Issue 4
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- Pages.184-188
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- 2000
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Regulation of HMG-CoA Reductase mRNA Stability by 25-hydroxycholesterol
- Park, Jae-Won (Department of Pharmacology, College of Medicine, Yonsei University) ;
- Oh, Seung-Min (Department of Pharmacology, College of Medicine, Yonsei University)
- Published : 2000.12.01
Abstract
HMG-CoA reductase is th rate-limiting enzyme of cholesterol biosynthesis. As intracellular levels of cholesterol should be regulated elaborately in response to external stimuli an internal needs, the expression of the HMG-CoA reductase gene is regulated intricately at several different levels from transcription to post-translational modification. In this study, we investigated the regulatory mechanism of HMG-CoA reductase gene expression at the post-transcriptional/pre-translational levels in a baby hamster kidney cell line, C100. when 25-hydroxycholesterol was added to cells cultured in medium containing 5% delipidized fetal bovine serum and 25