The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지

RT-PCR Detection of Citrus Tristeza Virus form Early Satsuma Nandarin and Yuzu in Cheju Island

  • Kim, Daehyun (Department Genetic Engineering, Sungkyunkwan University) ;
  • Jaewook Hyun (Citrus Experiment Station, Rural Development Administration) ;
  • Hyunsik Hwang (Department Genetic Engineering, Sungkyunkwan University) ;
  • Lee, Sukchan (Department Genetic Engineering, Sungkyunkwan University)
  • 발행 : 2000.02.01
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초록

Citrus tristeza virus (CTV) was identified form CTV-infected early satsuma mandarin (Citus unshiu) and yuzu (C.junos) by RT-PCR. The total RNAs were isolated from citrus bark and seaf tissues infected with CTV and reverse transcription was followed with primers designed for amplifying CTV coat protein gene. DNA fragments 738 bp were amplified by RT-PCR and these products were colned for sequence analysis. Based on the sequence analysis, this PCR product has 97% sequence homology to CTV (T-385) CP gene isolated from USA. RT-PCR assay for CTV detection was more sensitivity than ELISA assay which was done with anti-CTV CP antibody. This is the frist report about CTV identification in Cheju island Korea.

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