한국수정란이식학회지 (Journal of Embryo Transfer)
- 제15권2호
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- Pages.191-196
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- 2000
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
단순한정배양액 내의 Sodium Chloride 및 Macromolecules가 소 수정란의 체외발육에 미치는 영향
Effects of Sodium Chloride and Macromolecules in Chemically Defined Culture Medium on In Vitro Development of Bovine Embryos
초록
the present study was carried out to develop a completely defined culture system and determine if high NaCl concentrations in defined (PVA added) or semi-defined (BSA added) medium is toxic to bovine embryos. Oocytes from slaughterhouse ovaries were matured and fertilized in vitro. After 30 h of insemination, only 2-cell stage embryos were selected and cultured for this experiment. The culture media used were as follows : TLP(114 mM of NaCl) + BSA (3 mg/ml), TLP + PVA (1 mg/ml), mTLP(96 mM of NaCl) + BSA, mTLP + PVA. Six to ten embryos were placed into a 30