KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Supercritical Fluid Extraction of Physiologically Active materials from Agaricus blazei Fruiting Bodies

Agaricus blazei 자실체로부터 초임계 유체를 이용한 생리활성물질 추출공정

  • Published : 2000.08.01
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Abstract

The supercritical fluid extraction(SFE) technique was applied for the isolation and purification of nonpolar physiologically acitve mateials from Agaricus blazei fruiting bodies. the qualitative analysis of extract was accomplished by gas chromatography-mass spectrometer(GC-MS) and extract was determined as linoleic acid(cis-9 cis-12-octadecadienoic acid) In order to obtain the optimum operating conditions of supercritical fluid extraction process the various temperatures and pressures were applied for process operation. From the comparison of exraction efficiencies $50^{\circ}C and 200 kg_f/cm^2$ were determined as optimum conditions.

생리활성물질의 함유량이 많은 아가리쿠스버섯으로부터 초임계 유체 추출 기술을 이용하여 비극성 생리활성물질인 linoleic acid를 분리, 정제하였다. Gas chromatography - mass spectrometer를 이용하여 추출된 물질의 정성분석을 수행하여 추출된 물질은 약리작용 효과를 가지는 linoleic acid (cis-9, cis-12-octadecadienoic acid)로 분석되었다. 초임계 추출법을 이용한 아가리쿠스 버섯으로부터의 생리활성물질 추출공정의 최적화를 위하여 다양한 온도와 압력하에서 실힘을 수행하였으며 추출 효율을 비교한 결과 $50^{\circ}C, 200 kg_f/cm^2$의 최적 운전조건을 결정하였다.

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