KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Recovery of Plant Cell and Its Debris by pH Control

pH 조절에 의한 식물세포 및 세포조각 회수

  • 김진현 ((주)삼양제넥스 생명공학연구소)
  • Published : 2000.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

A novel recovery method was developed to obtain the plant cell and its debris from Taxus chinensis cell cultures By pH control of plant cell cultures plant cell and debris was precipitated. The best pH is between 1.8 and 2.2 to obtain the precipitate of the plant cell and debris. Also paclitaxel is stable in this acidic conditions. This method is very simple an efficient to recover the plant cell and debris from plant cell cultures.

Keywords

References

  1. Biotechnol. Bioeng. v.44 Kinetics of Taxol Production, Growth and Nutrient Uptake in Cell Suspensions of Taxus cuspidata Fett-Neto, A.G.;W.T. Zhang;F. DiCosmo
  2. Plant Science v.84 Factors Influencing the in vitro Production of Radiolabelled Taxol by Pacific Yew, Taxus brevifolia Strobel, G.A.;A. Stierle;F.J.G.M. Van Kuijk
  3. J. of Natural Products v.53 The Chemistry of Taxol, A Clinically Useful Anticancer Agent Kingston, D.G.I.;G. Samaranayake;C.A. Ivey
  4. Pharmaceutical Research v.10 Taxol and Related Taxanes. I.Taxanes of Taxus brevifolia Bark Rao, K.V.
  5. J. of Natural Products v.53 Taxus spp. Needles Contain amounts of Taxol Comparable to the Bark of Taxus brevifolia : Analysis and Isolation Witherup, E.M.;S.A. Look;M.W. Stasko;T.J. Ghiorzi;G.M. Muschik
  6. Pharmaceutical Research v.12 A New Large-Scale Process for Taxol and related taxanes from Taxus brevifolia Rao, K.V.;J.B. Hanuman,;C. Alvarez;M. Stoy;J. Juchum;R.M. Davies;R. Baxley
  7. Membrane Separations in Biotechnology (1st ed.) McGregor, W.C.
  8. U.S.Patent. 5,871,979 Method for Mass Production of Taxol by Semi-Continuous Culture with Taxus chinensis Cell Culture Choi, H.K.;T.L. Adams;R.W. Stahlhut;S.I. Kim;J.H. Yun;B.K. Song;J.H. Kim;S.S. Hong;H.S. Lee
  9. U.S.Patent, 5,900,979 Method for Purifying Taxol from Taxus Biomass Hong, S.S.;B.K. Song;J.H. Kim;C.B. Lim;H.S. Lee;K.W. Kim;I.S. Kang;H.B. Park
  10. Taxane Anticancer Agents (1st ed.) Georg, G.I.;T.T. Chen;I. Ojima;D.M. Vyas