Increase of Cell Concentration by the Automatic Addition of Glucose and Ammonium to an Alcohol Distillery Wastewater Reutilized for Cultivating a Baker's Yeast : Automatic Analysis and Control of Ammonium Concentration with an On-line Flow Injection Analysis System

알콜증류폐액을 이용한 빵효모배양에서 Glucose와 Ammonium의 자동첨가에 의한 증균 : 온라인 FIA 시스템에 의한 Ammonium의 자동분석 및 제어

  • Published : 2000.04.01

Abstract

Automatic addition of glucose and ammonium to an alcohol distille깨 wastewater and the control of them at low $\infty$ncentration were tried to efficiently increase the cell concentration of a baker's yeast c비tivated in that wastewater. Added g glucose was indirectly controlled to less than 116 mg/L by a method which used DO as control parameter. Ammonium was a automatically measured and controlled within the range of 7.0~27.7 mM by a homemade on-line system which adopted FIA a as measurement method. Maximum specific growth rate and biomass yield to glucose were $0.21 hr^{-1}$ and about 0.78 g/g, w which were significantly increased values in contrast to those of an experiment without ammonium control. Biomass yield to a ammonium was 11.3 gIg. Cell cone엉ntration could be increased from 2.6 g/L to 18.5 g/L by the add ion of glucose and a ammonium.

알콜증류폐액에 빵효모를 배양하는 중에 glucose와 ammon-ium을 자동첨가하여 균체량을 효율적으로 증가시킴으로써 폐액의 재이용성을 향상시키고자 하였다. Glucose는 DO를 제어파라미터로 하는 제어방법을 사용하여 116 mg/L 이하로 자동제어하였다. Ammonium의 경우에는 FIA를 이용한 온라인 자동계측제어장치와 프로그램을 이용하여 배양액의 ammon-ium 농도를 자동분석하고 제어하였으며, ammonium 농도를 류.0~27.7 mM의 낮은 농도범위로 유지시킬 수 있었다. Ammo-nium 농도를 제어한 본 실험의 최대비증식속도는 0.21 hr-1이었고, 대당균체수율은 약 0.78 g/g 으로서 ammonium 농도를 제어하지 않은 경우에 비하여 대당균체수율은 약 1.4배, 최대비증식속도는 1.2배 더 높아졌으므로 ammonium 제어에 의하여 균의 glucose 이용효율이 더욱 향상되었다. 대암모늄균체수율은 약 11.3 g/g 이었다. Glucose와 ammonium을 첨가함으로써 균체량을 18.5 g/L 까지 높일 수 있었으며, 이는 첨가하지 않은 경우보다 7.1배 더 높은 균체량에 해당한다. Glucose와 ammonium의 농도를 낮게 유지시킬 수 있었으므로 두가지 영양성분의 경제적인 공급이 가능하였다.

Keywords

References

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