Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Culture of Human Umbilical Vein Endothlial Cells Using 96-well Microplates and Position Effects on Cell Growth

  • Lee, Soohyun (Department of Microbial Engineering) ;
  • Insook Sohn (Department of Gynecolgy, Konkuk university, 93-1 Mojin-dong, Kwangjin-gu, Seoul 143-701 Korea) ;
  • Park, Myungjin (Department of Microbial Engineering, Laboratory of Cell Biology, Korea Cancer Center hospital, Seoul 139-705) ;
  • Park, Inchul (Department of Microbial Engineering, Laboratory of Cell Biology, Korea Cancer Center hospital, Seoul 139-705) ;
  • Youngsook Sohn (Laboratory of Cell Biology, Korea Cancer Center hospital, Seoul 139-705) ;
  • Seokil Hong (Laboratory of Cell Biology, Korea Cancer Center hospital, Seoul 139-705) ;
  • Taeboo Choe (Department of Microbial Engineering)
  • Published : 2000.06.01
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Abstract

When endothelial cells isolated isolated from human umbilical venis were cultred for 6dary using 96-well microplates, the final cell density in each was fiund not to be the same although the medium composition of each well was exactly the same. Cell growth in the wells located at the periphery of a microplate was low, while that in the central area of the plate was high. A possible cause for different rate of growth was proposed as the uneven concentration of oxygen in the culture medium of each well.

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References

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